Sodium carboxymethylcellulose cmc na

Cadmium acetate (CdAc₂), Pue (for in vivo studies), and poly L-lysine hydrobromide (L-glutamine) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium Carboxymethylcellulose (CMC-Na) and penicillin–streptomycin were purchased from Solarbio (Beijing, China). Neurobasal^TM medium, B-27 supplement, Dulbecco’s Modified Eagle’s Medium/Ham’s F-12 Nutrient Mixture (DMEM/F-12), and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). Rapamycin (Rap), Lyso-Tracker Red (LTR), Hoechst33342 staining, and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Beyotime (Shanghai, China). DQ-BSA-Green was purchased from Invitrogen (Carlsbad, CA, USA). Pue (for in vitro studies) was purchased from Selleck Chemicals (Houston, TX, USA). Bafilomycin A1 (Baf A1) was purchased from MedChem Express (Monmouth Junction, NJ, USA). All other chemicals and reagents used were of analytical grade.

BBR, Evans blue (EB), triphenyltetrazolium chloride (TTC), 4′,6-diamino-2-phenylindole (DAPI), and protease inhibitor cocktail were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carboxymethylcellulose (CMC-Na) was purchased from Solarbio Technology (Beijing, China). Lactate dehydrogenase (LDH) assay kit, creatine kinase (CK), malondialdehyde (MDA), superoxide dismutase (SOD), superoxide generation, IL-6, and myeloperoxidase (MPO) assay kits were purchased from Jiancheng Bioengineering Institute (Nanjing, China). TNF-α ELISA kit was purchased from R&D Corporation (Minneapolis, USA). Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay kit was obtained from Roche Molecular Biochemicals (Mannheim, Germany). BCA protein quantification kit was purchased from Merck Millipore Technology (Darmstadt, Germany). The primary antibodies against SIRT1, Ac-Foxo1, gp91^phox, caspase-3, Bcl-2, Bax, β-actin, and SIRT1 siRNA were purchased from Santa Cruz Biotechnology (CA, USA). Rabbit anti-goat, goat anti-rabbit, and goat anti-mouse secondary antibodies were purchased from the Zhongshan Company (Beijing, China).