The cells isolated from digested lungs were stained with biotin-conjugated antibody mixtures for lineage markers (CD4, CD5, CD8, CD11c, CD11b, CD19, NK1.1, Gr-1, TER119, FcεRI, and B220), Pacific blue-conjugated anti-Sca-1, PECy7-conjugated c-Kit (CD117), APC-conjugated anti-IL-7Rα (CD127), FITC conjugated anti-T1/ST2, APC-Cy7-conjugated anti-CD25, and PE conjugated anti-streptavidin, and analyzed using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany). Lin−Sca+c-Kit+IL-7R+CD25+ST2dim cells were identified as lung ILC2s [21 (link), 22 (link)]. The data were analyzed using FlowJo (TreeStar, Ashland, OR, USA). APC-Cy7-conjugated anti-CD25, Pacific blue-conjugated anti-Sca-1, biotin-conjugated anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-NK1.1, anti-Gr-1, anti-TER119, and anti-B220, and PE-conjugated anti-streptavidin antibodies were obtained from BD Biosciences (Franklin Lakes, NJ, USA). FITC-conjugated anti-T1/ST2 was from MD Bioscience (St Paul, MN, USA). APC-conjugated anti-IL-7Rα and biotin-conjugated anti-FcεRI antibodies were from BioLegend (San Diego, CA, USA). PECy7-conjugated c-Kit was from eBioscience (La Jolla, CA, USA). Biotin-conjugated anti-CD11c and anti-CD19 were from TONBO Biosciences (San Diego, CA, USA).
Fitc conjugated anti t1 st2
Manufactured by Miltenyi Biotec
Sourced in Germany
FITC conjugated anti-T1/ST2 is a monoclonal antibody that specifically binds to the T1/ST2 protein. T1/ST2 is a member of the interleukin-1 receptor family and is expressed on the surface of various cell types. The FITC (Fluorescein Isothiocyanate) conjugation allows for the detection and analysis of T1/ST2-positive cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Apc conjugated anti il 7rα, by Miltenyi Biotec (2 mentions)
Macsquant analyzer, by Miltenyi Biotec (2 mentions)
Anti ter119, by BD (2 mentions)
Anti gr 1, by BD (2 mentions)
Anti b220, by BD (2 mentions)
Biotin conjugated anti cd4, by BD (2 mentions)
Pe conjugated anti streptavidin, by BD (2 mentions)
Apc conjugated anti il 7rα, by BioLegend (2 mentions)
Biotin conjugated anti fcεri antibodies, by BioLegend (2 mentions)
Pecy7 conjugated c kit, by Thermo Fisher Scientific (2 mentions)
Anti cd8, by BD (2 mentions)
Anti cd11b, by BD (2 mentions)
Apc cy7 conjugated anti cd25, by BD (2 mentions)
Anti nk1, by BD (2 mentions)
Anti cd5, by BD (2 mentions)
Anti cd19, by Cytek Biosciences (1 mentions)
2 protocols using fitc conjugated anti t1 st2
1
Lung Innate Lymphoid Cell Type 2 Isolation
2
Identification and Analysis of Lung ILC2s
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Analyses of ILC2s. The cells isolated from digested lungs were stained with biotin-conjugated antibody mixtures for lineage markers (CD4, CD5, CD8, CD11c, CD11b, CD19, NK1.1, Gr-1, TER119, FcεRI, and B220), Paci c blue-conjugated anti-Sca-1, PECy7-conjugated c-Kit (CD117), APC-conjugated anti-IL-7Rα (CD127), FITC conjugated anti-T1/ST2, APC-Cy7-conjugated anti-CD25, and PE conjugated antistreptavidin, and analyzed using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany).
Lin -Sca + c-Kit + IL-7R + CD25 + ST2 dim cells were identi ed as lung ILC2s [21] [22] . The data were analyzed using FlowJo (TreeStar, Ashland, OR, USA). APC-Cy7-conjugated anti-CD25, Paci c blue-conjugated anti-Sca-1, biotin-conjugated anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-NK1.1, anti-Gr-1, anti-TER119, and anti-B220, and PE-conjugated anti-streptavidin antibodies were obtained from BD Biosciences (Franklin Lakes, NJ, USA). FITC-conjugated anti-T1/ST2 was from MD Bioscience (St Paul, MN, USA). APCconjugated anti-IL-7Rα and biotin-conjugated anti-FcεRI antibodies were from BioLegend (San Diego, CA, USA). PECy7-conjugated c-Kit was from eBioscience (La Jolla, CA, USA). Biotin-conjugated anti-CD11c and anti-CD19 were from TONBO Biosciences (San Diego, CA, USA).
Lin -Sca + c-Kit + IL-7R + CD25 + ST2 dim cells were identi ed as lung ILC2s [21] [22] . The data were analyzed using FlowJo (TreeStar, Ashland, OR, USA). APC-Cy7-conjugated anti-CD25, Paci c blue-conjugated anti-Sca-1, biotin-conjugated anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-NK1.1, anti-Gr-1, anti-TER119, and anti-B220, and PE-conjugated anti-streptavidin antibodies were obtained from BD Biosciences (Franklin Lakes, NJ, USA). FITC-conjugated anti-T1/ST2 was from MD Bioscience (St Paul, MN, USA). APCconjugated anti-IL-7Rα and biotin-conjugated anti-FcεRI antibodies were from BioLegend (San Diego, CA, USA). PECy7-conjugated c-Kit was from eBioscience (La Jolla, CA, USA). Biotin-conjugated anti-CD11c and anti-CD19 were from TONBO Biosciences (San Diego, CA, USA).
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