Anti occludin occ

Manufactured by Bioss Antibodies

Sourced in United States, China

Anti-occludin (OCC) is a primary antibody that recognizes the tight junction protein occludin. Occludin is a transmembrane protein that plays a crucial role in the formation and regulation of tight junctions between epithelial and endothelial cells. The Anti-occludin (OCC) antibody can be used to detect and study the expression and localization of occludin in various biological samples.

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Lab products found in correlation

Anti claudin 1, by Bioss Antibodies (2 mentions) Anti tubulin antibodies, by Bioss Antibodies (2 mentions) Anti zo 1, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Occludin (20 citations)

2 protocols using anti occludin occ

Western Blot Analysis of Jejunum Tight Junctions

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Expression of tight junction proteins of jejunum was determined by WB analysis, which was conducted as previously described (Meng (link)
et al., 2017 (link)). Primary antibodies used include anti-zonula occludens 1 (ZO-1) (1:2,000, Santa Cruz, USA), anti-occludin (OCC) (1:500, Bioss, China), anti-claudin-1 (1:500, Bioss, China) and anti-tubulin antibodies (1:5,000, Bioss, China). Finally, chemiluminescence detection was performed with western ECL reagent on a FluorChem M Fluorescent Imaging System (ProteinSimple, Santa Clara, CA, USA). Densitometry analysis was perform using Image J software (NIH, Bethesda, MD, USA).

Song M., Zhang F., Chen L., Yang Q., Su H., Yang X., He H., Ling M., Zheng J., Duan C., Lai X., Pan M., Zhu X., Wang L., Gao P., Shu G., Jiang Q, & Wang S. (2021). Dietary chenodeoxycholic acid improves growth performance and intestinal health by altering serum metabolic profiles and gut bacteria in weaned piglets. Animal Nutrition, 7(2), 365-375.

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Western Blot Analysis of Tight Junction Proteins

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Expression of tight junction (TJ) proteins in jejunum was determined by WB analysis which was conducted as previously described [25 (link)]. Proteins were detected by primary antibodies: anti-Tubulin antibodies (1:5000, Bioss, China), anti-Occludin (OCC) (1:500, Bioss, China), anti-ZO-1 (1:2000, Santa Cruz, USA) and anti-Claudin-1 (1:500, Bioss, China), and then incubated with the secondary antibodies (1:2000, Bioss, China). Finally, chemiluminescence detection was performed with western ECL reagent and band densities were quantified using a FluorChem M Fluorescent Imaging System (ProteinSimple, Santa Clara, CA, USA). The intensity of bands was analyzed and quantified by Image J Sofware (NIH, Bethesda, MD, USA).

Song M., Zhang F., Fu Y., Yi X., Feng S., Liu Z., Deng D., Yang Q., Yu M., Zhu C., Zhu X., Wang L., Gao P., Shu G., Ma X., Jiang Q, & Wang S. (2022). Tauroursodeoxycholic acid (TUDCA) improves intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets. Journal of Animal Science and Biotechnology, 13, 73.

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