Blood samples were centrifuged at 2500 rpm for 20 min; and serum was collected and stored at −80 °C. Leptin levels were assayed using a mouse-specific ELISA (Crystal Chem, Downers Grove, IL). For sensitivity studies, Leptin was provided by Dr. Parlow (Harbor-UCLA Medical Center, Torrance, CA) through the National Hormone and Peptide Program. Mouse FGF21 and human FGF21 proteins were measured by ELISA (R & D Systems, MN). Analysis of intact FGF21 was performed by Lilly Research Laboratories by their in-house ELISA and also confirmed by a commercially available ELISA kit (F2131-K01; Eagle Biosciences, Nashua, NH). Plasma levels for insulin like growth factor−1 (IGF-1) were determined using Quantikine ELISA kit for mouse/rat IGF-1 (MG100; R&D Systems, MN).
Quantikine elisa kit for mouse rat igf 1 mg100
Manufactured by R&D Systems
Sourced in United States
The Quantikine ELISA kit for mouse/rat IGF-1 (MG100) is a quantitative sandwich enzyme immunoassay designed to measure mouse or rat insulin-like growth factor 1 (IGF-1) levels in cell culture supernates, serum, and plasma. The assay employs an antibody specific for mouse/rat IGF-1 coated on a microplate. Standards and samples are pipetted into the wells, and any IGF-1 present is bound by the immobilized antibody.
Automatically generated - may contain errors
2 protocols using quantikine elisa kit for mouse rat igf 1 mg100
1
Quantification of Metabolic Biomarkers
2
Metabolic Profile Analysis from Murine Serum and Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected on ice, heparinized and spun at room temperature before storage of plasma at 4°C or allowed to clot and spun at 4°C before flash-freezing of serum in liquid nitrogen. Serum metabolites were measured by small-scale enzymatic assay for glucose (1070; Stanbio Laboratory, Boerne, TX) or using a standard automated glucose monitor. Serum insulin levels were determined by an ultrasensitive mouse-ELISA (Crystal Chem, Downers Grove, IL). Plasma levels for insulin like growth factor -1 (IGF-1) was determined using Quantikine ELISA kit for mouse/rat IGF-1(MG100; R&D Systems, MN, USA). Radioimmunoassay kit for glucagon estimation (GL-32K; Millipore, Billerica, MA) was used. For glucagon, blood samples were collected in tubes containing aprotinin (1 g/ml, Bayer Healthcare, Berkeley, CA), and the serum was stored at -80°C before radio-immunoassay. Serum amylase levels were analyzed using Biovision assay kit (K711-100; Biovision, Milpitas, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!