1 mL of blood was drawn by using a 22-gauge syringe from the abdominal region of the ascending aorta from each mouse under pentobarbital anaesthesia before euthanasia. Samples were centrifuged, allowing the isolation of sera. Sera were snap frozen in liquid nitrogen and stored at −80 °C. Fifteen microlitres of sera were isolated for Milliplex assay (Millipore Milliplex MAP multiplex panels; Merck Millipore, Burlington, Massachusetts, USA). Analytes probed for included a number of predetermined proinflammatory cytokines and chemokines, including: IL-1b, 2, 10, 12, 15, TNF, and MCP-1. Analysis was performed according to the manufacturer’s instructions.
Millipore milliplex map multiplex panels
Manufactured by Merck Group
Sourced in United States, Germany
The Millipore Milliplex MAP multiplex panels are a series of lab equipment used for the quantitative analysis of multiple analytes in a single sample. The panels utilize magnetic beads coated with specific capture antibodies to detect and measure the concentrations of various proteins, cytokines, and other biomolecules simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using millipore milliplex map multiplex panels
1
Serum Cytokine Profiling in Mice
2
Quantification of IL-13 and Periostin in Tears
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Tears exceeding 20 mL were collected into Nunc minisorp tubes (Nunc, Roskilde, Denmark) using a micropipette, as previously described. 28 IL-13 in tears was quantified by Multiplex assay kits for human IL-13 (Millipore Milliplex MAP multiplex panels; Merck Millipore, Darmstadt, Germany). Periostin in tears and serum was quantified by ELISA kits for human periostin that we established (clones nos. SS18A and SS17B). 29
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