Macrophages were transfected with 60 pmol SAMHD1-specific siRNA using GenMute™ siRNA Transfection Reagent for Primary Macrophages (SignaGen Laboratories) according to the manufacturer’s instructions. Cells were again transfected on day 4 and day 8 after the initial transfection (total of 3 transfections). Cells were ready for infection or lysis 14 days after the initial transfection. The following siRNAs were used: SAMHD1 silencer select siRNA (Thermo Fisher Scientific); Silencer® Select Negative Control No. 1 siRNA (Life Technologies). Lyophilized siRNAs were re-hydrated in 1x siRNA Buffer (Dharmacon) at a concentration of 20nM and stored at −20°C.
Samhd1 silencer select sirna
Manufactured by Thermo Fisher Scientific
The SAMHD1 silencer select siRNA is a laboratory tool designed to temporarily silence the expression of the SAMHD1 gene. SAMHD1 is a cellular enzyme that plays a role in regulating the levels of certain nucleotides. This siRNA can be used to investigate the functional effects of SAMHD1 downregulation in cellular and molecular biology research.
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Lab products found in correlation
2 protocols using samhd1 silencer select sirna
1
SAMHD1 Knockdown in Macrophages
2
SAMHD1 Silencing in Macrophages
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Macrophages were transfected with 60 pmol SAMHD1-specific siRNA using GenMute™ siRNA Transfection Reagent for Primary Macrophages (SignaGen Laboratories) according to the manufacturer's instructions. Cells were again transfected on day 4 and day 8 after the initial transfection (total of 3 transfections). Cells were ready for infection or lysis 14 days after the initial transfection. The following siRNAs were used: SAMHD1 silencer select siRNA (Thermo Fisher Scientific); Silencer® Select Negative Control No. 1 siRNA (Life Technologies). Lyophilized siRNAs were re-hydrated in 1x siRNA Buffer (Dharmacon) at a concentration of 20nM and stored at -20°C.
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