Immunocytochemistry and alkaline phosphatase (AP) (Millipore #SCR004) staining were performed as previously indicated [18 (link)]. Primary antibodies included SSEA1, SSEA4, TRA-1-60 and TRA-1-81 from the ESC characterization tool (all 1:100, Millipore #SCR004), NANOG (1:100, Abcam #ab62734), Smooth Muscle Actin (SMA) (1:100, Dako Cytomation #M0851), Alpha Feto Protein (AFP) (1:100, Sigma #WH0000174M1), SOX17 (1:50, R&D #AF1924), PAX6 (1:300, Covance #PRB-278P), NESTIN (1:200, Chemicon #MAB5326), TUJ-1 (1:1000, Sigma #T8660). Secondary antibodies were conjugated with either Alexa 488 or Alexa 594 (Invitrogen #A11001, A11055, A21201, A21468, A11005, A21442). Coverslips were mounted using Dako fluorescent mounting medium (Dako #S3023) and visualized using a confocal microscope LSM 510 (Zeiss).
M0851
Manufactured by Merck Group
The M0851 is a laboratory equipment product manufactured by Merck Group. It serves as a core component for various scientific applications. The device's primary function is to perform standardized measurements and data collection tasks within a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 510, by Zeiss (2 mentions)
Ab62734, by Abcam (1 mentions)
A11005, by Thermo Fisher Scientific (1 mentions)
A11055, by Thermo Fisher Scientific (1 mentions)
S3023, by Agilent Technologies (1 mentions)
Scr004, by Merck Group (1 mentions)
Scr004, by Abcam (1 mentions)
Wh0000174m1, by R&D Systems (1 mentions)
A21201, by Thermo Fisher Scientific (1 mentions)
Mab5326, by Merck Group (1 mentions)
Prb 278p, by Merck Group (1 mentions)
A21468, by Thermo Fisher Scientific (1 mentions)
T8660, by Merck Group (1 mentions)
A11001, by Thermo Fisher Scientific (1 mentions)
Normal goat serum (ngs), by Agilent Technologies (1 mentions)
α actinin, by Merck Group (1 mentions)
Hoechst, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Carl Roth (1 mentions)
2 protocols using m0851
1
Immunocytochemistry and Alkaline Phosphatase Staining
2
Immunofluorescence Analysis of Cytoskeletal Proteins
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Cells were fixed with 4% paraformaldehyde (Roth, Karlsruhe, Germany), blocked with 5% normal goat serum (Dako, Santa Clara, CA, USA) and permeabilized with 0.1% Triton X-100. Primary antibodies against vimentin (Abcam #ab27608), α-smooth muscle actin (Dako #M0851) or α-actinin (Sigma #A7811) were incubated overnight at 4 °C. The secondary antibody was Alexa488-conjugated (Invitrogen #A-11008 and #A-11001), and nuclei were stained with Hoechst (Invitrogen #H3570). Photomicrographs were taken with the EVOS cell imaging system, and positive cells were counted with ImageJ software.
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