Anti mitofusin 2

Oligomycin (O4876), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) (C2920), antimycin A (A8674), rotenone (R8875), carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (C2759), bafilomycin A1 (B1793), sodium pyruvate (P5280), and glucose (G7021) were purchased from Sigma-Aldrich (St. Quentin Fallavier, France). MitoTracker Deep Red (M22426) and l-glutamine (25030-024) were purchased from ThermoFisher Scientific (Illkirch, France). Cyto-ID (ENZ-51031) was purchased from Enzo Life Sciences (Villeurbanne, France). Anti-Mitofusin 2 (rabbit, 9482), anti-Grp75 (rabbit, 3593), anti-LC3B (rabbit, 2775), and anti-Myc (mouse, 2276) were from Cell Signaling Technology (Saint Quentin Yvelines, France). Anti-Tom20 (rabbit, sc-11415), Anti-Tom20-AF488 (rabbit, sc-17764), anti-Parkin (mouse, sc-32282), and anti-Ero1-Lα (mouse, sc-365526) were from Santa Cruz Biotechnology (Heidelberg, Germany). Anti-VDAC1 (mouse, ab14734), anti-Parkin (rabbit, ab15954), and anti-Beclin-1 (mouse, ab114071) were from Abcam (Paris, France). Anti-KDEL (mouse, ADI-SPA-827-D) was from Enzo Life Sciences. Anti-PACS-2 (rabbit, 19508-1-AP) was from Proteintech (Manchester, UK), and Anti-PACS-2 (rabbit, clone 18143) was previously reported [9 (link)]. Anti-IP3R1 (rabbit, 07-1213) was from Merck Millipore (Molsheim, France). Anti-β-actin (mouse, A2228) and anti-P62 (rabbit, P0067) were from Sigma-Aldrich.

AICAR (5-Aminoimidazole-4-carboxamide ribonucleotide), rapamycin, diclofenac, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) and collagenase were purchased from Sigma Aldrich. Acetaminophen was purchased from Bronson & Jacobs (Villawood, Australia). MitoTracker green, tetramethylrhodamine ethyl ester (TMRE), Hoechst 33342 and ATP determination kit were purchased from Invitrogen. Anti-Mitofusion-1 and anti-Tom20 antibodies were from Santa Cruz Biotechnology. Anti-Mitofusin-2, anti-AMPK alpha, anti-Phospho-AMPK alpha (Thr172), anti-ACC (Acetyl-CoA Carboxylase), anti-phospho-ACC (ser79), anti-Drp1, anti-phospho-Drp1 (ser616), anti-LC3 and anti-Parkin antibodies were from Cell Signaling Technology. Anti-OPA1 antibody was from BD Transduction Lab. Mouse anti-ABCB1 (C219) antibody was from Covance Research and rabbit anti-occludin antibody was purchased from Invitrogen. Secondary HRP-conjugated goat anti-rabbit and anti-mouse IgG were bought from Jackson ImmunoResearch. Anti-rabbit Alexa fluro 488 and anti-mouse CY3 antibodies were from Invitrogen. Clarity^TM ECL western blotting substrate was from Bio-Rad.