CircFOXO3 probes were designed and synthesized by RiboBio. The probe signals were detected with a fluorescence in situ hybridization (FISH) kit (RiboBio) according to the manufacturer’s instructions.
Circfoxo3 probes
Manufactured by RiboBio
CircFOXO3 probes are designed for the detection and quantification of the circular RNA FOXO3. These probes enable the specific and sensitive measurement of CircFOXO3 expression levels in various biological samples.
Automatically generated - may contain errors
2 protocols using circfoxo3 probes
1
FISH detection of CircFOXO3 probes
2
FOXO3 Circular RNA Expression in Cardiomyocytes
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Cell culture. Human cardiomyocytes (AC16; American Type Culture Collection) were cultured at 80% confluency in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.). Cells were incubated at 37˚C with 5% CO 2 .
RNA Fluorescence in Situ Hybridization (FISH). circFOXO3 probes were designed and synthesized by Guangzhou RiboBio Co., Ltd. The probe signals were detected with a FISH kit (Guangzhou RiboBio Co., Ltd.) according to the manufacturer's instructions. Briefly, 5x10 4 AC16 cells were fixed in 4% paraformaldehyde for 1 h at room temperature. Pre-hybridization were conducted with 200 µl pre-hybridization buffer at 37˚C for 30 min. Then, 0.5 µM circFOXO3 FISH Probe Mix or controls (U6 and 18S) were incubated overnight at 37˚C. Fluorescent microscopy (magnification, x400) were used to detect the signal.
Radiation treatment. Cells in culture were irradiated with an irradiator (Gammacell 3000 Elan; Atomic Energy of Canada Ltd.) using a 137Cs source at a dose rate of 6 Gy.
RNA Fluorescence in Situ Hybridization (FISH). circFOXO3 probes were designed and synthesized by Guangzhou RiboBio Co., Ltd. The probe signals were detected with a FISH kit (Guangzhou RiboBio Co., Ltd.) according to the manufacturer's instructions. Briefly, 5x10 4 AC16 cells were fixed in 4% paraformaldehyde for 1 h at room temperature. Pre-hybridization were conducted with 200 µl pre-hybridization buffer at 37˚C for 30 min. Then, 0.5 µM circFOXO3 FISH Probe Mix or controls (U6 and 18S) were incubated overnight at 37˚C. Fluorescent microscopy (magnification, x400) were used to detect the signal.
Radiation treatment. Cells in culture were irradiated with an irradiator (Gammacell 3000 Elan; Atomic Energy of Canada Ltd.) using a 137Cs source at a dose rate of 6 Gy.
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