Bsm 33187m

Manufactured by Bioss Antibodies

Sourced in China

The Bsm-33187M is a laboratory equipment product designed for scientific research. It is a multi-purpose device that can be used in various applications. The core function of the Bsm-33187M is to perform specific tasks required in a research laboratory setting. No further details can be provided without the risk of interpretation or extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 546, by Thermo Fisher Scientific (1 mentions) Vimentin, by Bioss Antibodies (1 mentions) Goat anti rabbit igg cy3, by Bioss Antibodies (1 mentions) Bs 0295g cy3, by Bioss Antibodies (1 mentions) Goat anti mouse igg fitc, by Bioss Antibodies (1 mentions) Bs 0296g fitc, by Bioss Antibodies (1 mentions) α sma, by Bioss Antibodies (1 mentions) Fluorescent microscope, by Olympus (1 mentions)

2 protocols using bsm 33187m

Quantifying Vessel Morphology via IHC

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For immunohistochemical analysis, ethanol was used to gradientlly dehydrate paraffin-embedded tissues. Antigen retrieval is then performed and incubated with the corresponding antibody including anti-CD31 (1:500, Aabcam, ab28364) and anti-α-SMA (1:200, Bioss Antibodies, bsm-33187M). For immunofluorescence of Integrin β1 and CD31, anti-rabbit IgG secondary antibody Alexa Fluor 546 conjugate (Invitrogen, 1:500, A11008) was used. For statistical analysis, 10 fields of view at 20x were selected for each group, and the number of vessels and vessel area were quantified using Image J. The short diameter of each vessel was also measured to characterize vessel maturation.

Wei Z., Lei M., Wang Y., Xie Y., Xie X., Lan D., Jia Y., Liu J., Ma Y., Cheng B., Gerecht S, & Xu F. (2023). Hydrogels with tunable mechanical plasticity regulate endothelial cell outgrowth in vasculogenesis and angiogenesis. Nature Communications, 14, 8307.

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Immunofluorescence Analysis of Isolated CAFs and NFs

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The isolated CAFs or NFs were fixed in 4% paraformaldehyde. After permeabilization in 0.2% Triton X-100 and blocking in goat serum, the cells were probed with primary antibodies α-SMA (1:500, bsm-33187M, Bioss, Beijing, China) and vimentin (1:100, bsm-0756R, Bioss) overnight at 4°C. Subsequently, Goat Anti-Mouse IgG/FITC (1:100, bs-0296G-FITC, Bioss) and Goat Anti-rabbit IgG/Cy3 (1:100, bs-0295G-Cy3, Bioss) were applied. After nuclear counterstaining with DAPI, the cells were observed using a fluorescent microscope (Olympus, Tokyo, Japan).

Fan J.T., Zhou Z.Y., Luo Y.L., Luo Q., Chen S.B., Zhao J.C, & Chen Q.R. (2021). Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia (New York, N.Y.), 23(7), 692-703.

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