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2 protocols using anti grp94

1

Antibody Sources for GFP, RFP, and PrP-A

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Rabbit antisera for GFP and RFP were made as described (Stefanovic and Hegde, 2007 ) and rabbit antisera for Tmp21 was made by immunizing rabbits with a peptide corresponding to the cytoplasmic tail (CLRRFFKAKKLIE) conjugated to keyhole limpet hemacyanin via the terminal cysteine. The PrP-A antibody was previously described (Rane et al., 2004 (link)). The antibody against TCR-α, A2B4, (gift from Carolyn Suzuki) was previously described (Suzuki et al., 1991 (link)). CHOP and XBP-1 antibodies were gifts from Linda Hendershot.
Purchased antibodies included anti-calnexin (StressGen SPA-860), anti-Grp94 (StressGen SPA-850), anti-ERGIC53 (Santa Cruz SC-66880), anti-alpha tubulin (Sigma T6199), anti-GAPDH (Santa Cruz SC-20357), Cy5-conjugated anti-rabbit IgG (Jackson ImmunoResearch 111-175-003) and Alexa 488 goat anti-mouse Ab (Invitrogen A11029).
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2

Comprehensive Antibody Panel for Protein Analysis

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Source of specific antibodies: anti-αB-crystallin (mouse, 1:2000), anti-Hsp 20 (rabbit,1:10,000), anti-S16 Hsp-20 (rabbit, 1:500), anti-Hsp27 (rabbit, 1:2000), anti-S82 Hsp27 (rabbit, 1:2000), anti-S59 αB-crystallin (rabbit, 1:2000) and anti-S45 αB-crystallin (rabbit, 1:1000) from Abcam; anti GRP78 (rabbit, 1:500), anti-CASQ2 (rabbit, 1:2000) and anti-CRT (rabbit, 1:1000) from Thermo; anti Bcl-2 (rabbit, 1:1000) and anti-CNX (rabbit, 1:1000) from Santa Cruz; anti-Actin (rabbit, 1:1000), anti-STIM1 (rabbit, 1:2000), anti-TRPC3 (rabbit, 1:500), anti-Orai1 (rabbit, 1:500), anti-EDEM1 (rabbit, 1:1000) and anti-Herp (rabbit, 1:500) from Sigma; anti-Beclin1 (rabbit, 1:5000) and anti-HRD1 (1:1000) from Novus Biologicals; anti-GRP94 (rabbit, 1:1000) from Stressgen; mono- and polyubiquitinated conjugates (mouse, 1:1000) from EnzoLifeScience. Anti Derlin-1 (mouse, 1:1000) was a generous gift of Doriana Sandonà.
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