Fitc conjugated goat antibody to rabbit igg

Mouse skin frozen sections were fixed with acetone, washed in 1X PBS, blocked with 5% goat serum/1% BSA for 30 minutes, and incubated with anti-MMP-9 polyclonal antibody (ABBIOTEC, San Diego, CA) as a primary antibody (diluted 1:200). FITC-conjugated goat antibody to rabbit IgG (Molecular Probes, Grand Island, NY) was used as a secondary antibody (diluted 1:1000) and was incubated for 40 minutes. For FcεRI detection, we used anti-mouse FcεRI PE (eBioscience, San Diego, CA). We mounted sections in ProLong Anti-Fade reagent (Molecular Probes) with 4’, 6-diamidino-2-phenylindole (DAPI). After overnight incubation at 4°C in the dark, images were obtained using a Zeiss LSM510 laser scanning confocal microscope coupled with an Axiovert 100 inverted stage microscope.