Phospho sting ser365 d8f4w rabbit mab

BX795 was purchased from Invivogen. CAY10576, BAY 11–7082, and Ruxolitinib were from Selleck Chemicals. Bortezomib and carfilzomib were from Cayman Chemical. Sulforaphane, cycloheximide, actinomycin D, oligomycin, and antimycin were obtained from Sigma-Aldrich. Mouse monoclonal Ngly1 antibody was a gift from A. Zuberi (Jackson Laboratories). Other antibodies used as follows: anti-STING (13647; Cell Signaling, 1:1,000 dilution), phospho-STING (Ser365; D8F4W) Rabbit mAb (72971; Cell Signaling; 1:1,000 dilution), anti-cGAS (31659; Cell Signaling; 1:1,000 dilution), anti–RIG-I (3743; Cell Signaling; 1:1,000 dilution), anti-MDA5 (5321; Cell Signaling; 1:1,000 dilution), anti-tubulin (B-5-1-2; Sigma-Aldrich; 1:20,000 dilution), anti-HMGB1 (ab18256; Abcam; 1:2,000 dilution), anti-GAPDH (2118; Cell Signaling; 1:2,000 dilution), anti-SQSTM1/p62 (ab56416; Abcam; 1:1,000 dilution), anti-phosphorylated p70 S6K (Thr389; 9205; Cell Signaling; 1:1,000 dilution), anti-p70 S6K (2708; Cell Signaling; 1:1,000 dilution), anti–phospho-ATM (Ser1981) antibody (clone 10H11.E12; EMD Millipore; 1:1,000 dilution), phospho-Histone H2A.X (Ser139) antibody (2577; Cell Signaling; 1:1,000 dilution), Nrf1 (8052; Cell Signaling; 1:1,000 dilution), Nrf2 (12721; Cell Signaling; 1:1,000 dilution).

UNC2250 was purchased from Adooq Bioscience (Nanjing, China). Oxaliplatin and irinotecan were purchased from MedChemexpress (Wuhan, China). Cytometric Beads for cytokine detection were purchased from BD Bioscience (Shanghai, China). BD Biosciences (Shanghai, China). DiD was purchased from Beyotime Biotechnology (Shanghai, China). Granulocyte/macrophage-colony stimulating factor (GM-CSF) was purchased from Dakewe (Shenzhen, China). Macrophage-colony stimulating factor (M-CSF) was purchased from Abcolonal (Wuhan, China), NGS™ dsDNA HS Assay Kit was purchased from Wuhan ABP-Biosciences Co., Ltd. (Wuhan, China). Gibco DMEM medium and Gibco RPMI 1640 medium were purchased from Thermo Fisher Scientific Co., Ltd. (Shanghai, China). Anti-γ H2A.X (phospho S139) antibody was purchased from Abcam (Shanghai, China). STING (D2P2F) Rabbit mAb was purchased from Cell Signaling Technology (Shanghai, China). Phospho-STING (Ser365) (D8F4W) Rabbit mAb was purchased from Cell Signaling Technology (Shanghai, China).