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2 protocols using antibody to lpl sc 16657 goat

1

Immunoblotting and Immunofluorescence Reagents

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Antibody to LPL (SC-16657; Goat) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies to GAPDH and TNF-α receptor 1 (TNFR1) were purchased from R & D Systems (Minneapolis, MN). Protein estimation reagent, molecular weight standards, and PAGE reagents were bought from Bio-Rad (Hercules, CA). Cy2- and Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). HRP-conjugated secondary antibodies for immunoblotting and the phosphoserine (p-Serine) antibody were purchased from Abcam (Cambridge, MA). Mounting solutions for mounting of coverslips were purchased from Thomas Scientific (Swedesboro, NJ) or Vector Labs (Burlingame, CA). Rhodamine-phalloidin and all other chemicals were purchased from Sigma (St. Louis, MO).
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2

Comprehensive Antibody Source Protocol

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Antibody to LPL (SC-16657; Goat) was bought from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies to GAPDH and TNF-α receptor 1 (TNFR1) were purchased from R & D Systems (Minneapolis, MN). Protein estimation reagent, molecular weight standards for proteins, and PAGE reagents were bought from Bio-Rad (Hercules, CA). Molecular weight markers have also been bought from Promega (Madison, WI). Cy2- and Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch (West Grove, PA). HRPconjugated secondary antibodies for immunoblotting and phosphoserine (p-Serine) antibody were bought from Abcam (Cambridge, MA). Mounting solutions for mounting of coverslips were bought from Thomas Scientific (Swedesboro, NJ) or Vector Labs (Burlingame, CA). Rhodaminephalloidin and all other chemicals were purchased from Sigma (St. Louis, MO).
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