Plate Count Agar (PCA), Rose Bengal Agar (RBA), Man Rogosa Sharpe (MRS) broth, pepsin, bile extract, and dextran (DX) of 20 kDa molecular weight were purchased from Sinopharm Chemical Reagent Co., ltd. (Shanghai, China). Whey protein isolate (WPI) was purchased from Hilmar Ingredients (California, USA). Eugenol was purchased from Aladdin (Shanghai, China). Fresh mature ‘Fuji’ apples (harvested in the fall of 2021) were purchased from a supermarket in Shaanxi province.
Rose bengal agar
Manufactured by Sinopharm
Sourced in China
Rose Bengal Agar is a microbiological culture medium used for the selective isolation and enumeration of fungi, particularly yeasts and molds, from food, soil, and other environmental samples. It contains Rose Bengal, a staining agent that inhibits the growth of fast-growing bacteria and restricts the size of fungal colonies, making it easier to enumerate and identify different fungal species.
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Lab products found in correlation
2 protocols using rose bengal agar
1
Fuji Apple Preservation with Antimicrobials
2
Aerobic Bacteria and Yeast&Mold Detection in Pitaya Juice
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The detection of viable total aerobic bacteria (AB) and Y&M in clear red pitaya juice followed the method described by Liao et al. [17 (link)]. Samples were serially diluted at 10 times gradient with 0.85% sterile saline solution, and 1 mL of each dilution was spread on duplicate sterile petri dishes containing the Nutrient agar (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) and the Rose bengal agar (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), to enumerate residual cultivable AB and Y&M cells, respectively. The numbers of AB and Y&M were counted after incubation at 37 ± 1 °C for 48 ± 2 h, and at 28 ± 1 °C for 72 ± 2 h. The limit of plate counting detection is 1 CFU/mL.
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