HEK293T, HEK293, and SH‐SY5Y cells were purchased from Cell Bank in the Chinese Academy of Sciences Shanghai. The HT22 mouse hippocampal neuronal cells were a gift from Prof. Lihong Jia (China Medical University). HEK293T, HEK293, and HT22 cells were cultured in high‐glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with fetal bovine serum (FBS; 10%, CLARK, Australia), penicillin (100 U), and streptomycin (100 μg/mL) in 5% CO2 at 37°C. SH‐SY5Y cells were cultured in high‐glucose DMEM/F12 (Genview, GF3105) supplemented with 10% FBS, penicillin (100 U), and streptomycin (100 μg/mL) in 5% CO2 at 37°C.
Dmem f12
Manufactured by Genview
Sourced in United States
DMEM/F12 is a culture medium used for the in vitro cultivation of cells. It is a mixture of Dulbecco's Modified Eagle's Medium and Ham's F-12 Nutrient Mixture, providing a balanced formulation of nutrients, vitamins, and salts to support cell growth and proliferation.
Automatically generated - may contain errors
2 protocols using dmem f12
1
Cell Culture Protocols for Neuroscience Research
2
Isolation and Culture of Rat ADSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The ADSCs were carefully isolated from rat inguinal fat pads, dissected and minced under aseptic conditions. Subsequently, the tissue was enzymatically dissociated at 37°C for 60 min using 0.1% collagenase I (Invitrogen; Thermo Fisher Scientific, Inc.). The dissociated cells were cultured in Dulbecco's modified Eagle's medium/F12 (DMEM/F12; Genview, Tallahassee, FL, USA) containing 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Beit Haemek, Israel) and 1% (v/v) penicillin/streptomycin solution for 48 h at 37°C in 5% CO2. The non-adherent cells were removed on replacement of the medium every 2–3 days when the cells reached 80% confluence.
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