Cesarean section was performed under spinal anesthesia. Women were in a fasting state and did not receive intravenous glucose infusion during the surgery. We did not register individually the exact time of the last meal before surgery, but the median (10th-90th percentile) duration of fasting from 2 am until surgery start was 8 hours and 15 minutes (7-10 hours 30 minutes). During surgery, blood samples were obtained from the maternal antecubital vein, radial artery and from the UA and UV immediately after delivery of the baby, but before the umbilical cord was clamped. 22 The sampling procedure took 2-4 minutes. Glucose, insulin, and triglycerides were measured by an accredited laboratory (Department of Medical Biochemistry, Oslo University Hospital) using the hexokinase/glucose-6-phosphate dehydrogenase enzymatic in vitro test (Roche, Mannheim, Germany).
Hexokinase glucose 6 phosphate dehydrogenase enzymatic in vitro test
Manufactured by Roche
Sourced in Germany
The Hexokinase/glucose-6-phosphate dehydrogenase enzymatic in vitro test is a laboratory equipment product. It is used to measure the activity of the enzymes hexokinase and glucose-6-phosphate dehydrogenase.
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Lab products found in correlation
2 protocols using hexokinase glucose 6 phosphate dehydrogenase enzymatic in vitro test
1
Maternal Metabolic Profile During Cesarean
2
Venous Blood Sampling for Glucose Analysis
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After each ultrasound examination, pre- and postprandial venous blood samples were collected in sodium heparin vacutainers, kept on ice, and centrifuged within 20 min at 6°C (2500 ×g, 20 min). The supernatants were carefully removed and stored at ‒80°C. Glucose was measured using the hexokinase/glucose-6-phosphate dehydrogenase enzymatic in vitro test (Roche, Mannheim, Germany), performed by an accredited laboratory at the Department of Medical Biochemistry, Oslo University Hospital–Rikshospitalet.
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