Pe labelled fluorescent streptavidin

Manufactured by BioLegend

PE-labelled fluorescent streptavidin is a protein complex consisting of the protein streptavidin conjugated with the fluorescent dye phycoerythrin (PE). Streptavidin is a tetrameric protein that binds strongly and selectively to the small molecule biotin. The PE label provides a fluorescent signal that can be detected and quantified using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Biotinylated peptide, by GenScript (2 mentions)

Spelling variants of this product

Streptavidin-PE (106 citations)

2 protocols using pe labelled fluorescent streptavidin

Identifying Targetable Epitopes Using Bioinformatics and Tetramerization

Cited 1 time

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In order to determine which peptide would be best targeted by an
antibody, the amino acid sequence for SDCBP was run through two epitope
prediction tools (Bepipred Linear Epitope Prediction 2.0 and ABCPred) to
determine predicted epitopes. Predicted targetable extracellular domains were
chosen as the peptides for tetramer analysis. We tetramerized two biotinylated
peptides (GenScript) contained in the extracellular domain of syndecan binding
protein (SDCBP) using PE-labelled fluorescent streptavidin (BioLegend, 405203)
and used flow cytometry to determine the percent of specific antigen-reactive
immortalized B cells which are secreting antibodies against the target molecule
from each of the six immortalized B cell lines, using the protocol previously
described.^{5 (link)} We then
used fluorescence-activated cell sorting with EEEIRANVAVVSGAPL peptide to sort
endometrioid carcinoma-derived immortalized B cells specific for SDCBP from the
pool of immortalized B cells.

Handley K.F., Mehta S., Martin A.L., Biswas S., Maharaj K., Nagy M.Z., Mine J.A., Cortina C., Yu X., Sprenger K., Mandal G., Innamarato P., Powers J.J., Harro C.M., Chaurio R.A., Anadon C.M., Shahzad M.M., Flores I, & Conejo-Garcia J.R. (2023). Actionable spontaneous antibody responses antagonize malignant progression in ovarian carcinoma. Gynecologic oncology, 173, 114-121.

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Identifying Antibody Targeting Peptides

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In order to determine which peptide would be best targeted by an antibody, the amino acid sequence for SDCBP was run through two epitope prediction tools (Bepipred Linear Epitope Prediction 2.0 and ABCPred) to determine predicted epitopes. Predicted targetable extracellular domains were chosen as the peptides for tetramer analysis. We tetramerized two biotinylated peptides (GenScript) contained in the extracellular domain of syndecan binding protein (SDCBP) using PE-labelled fluorescent streptavidin (BioLegend, 405203) and used flow cytometry to determine the percent of specific antigen-reactive immortalized B cells which are secreting antibodies against the target molecule from each of the six immortalized B cell lines, using the protocol previously described. 5 We then used fluorescence-activated cell sorting with EEEIRANVAVVSGAPL peptide to sort endometrioid carcinoma-derived immortalized B cells specific for SDCBP from the pool of immortalized B cells.

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