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Ds ri1 epifluorescence microscope

Manufactured by Nikon
Sourced in United States, Japan

The DS-Ri1 is an epifluorescence microscope manufactured by Nikon. It is designed to capture high-quality fluorescence images. The microscope features a high-resolution camera and specialized optical components to enable fluorescence imaging applications.

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2 protocols using ds ri1 epifluorescence microscope

1

Immunocytochemical Analysis of PDGFR in Tissues

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Thick transverse cross-sections (8 µm) of tissues were obtained using a cryostat (2800 FrigocutN, Reichert Jung, Heidelberg, Germany) equilibrated at -30 and -20°C for adipose tissue and muscle, respectively. Sections were mounted on silane-coated slides for enhanced adhesion. Immunocyto-localization of PDGFR marker was carried out using a standard procedure. Briefly, after incubation in 2% bovine serum albumin (BSA; Sigma, Saint-Quentin Fallavier, France) to prevent non-specific binding, sections were incubated in PBS/0.1% BSA for 45 min at room temperature with the following primary mouse antibodies: anti-PDGFR and anti-laminin complex (Interchim, Montluçon, France). Nuclei were counterstained with 4'-6-diamidino-2-phenylindole (DAPI; Dako, Trappes, France). No significant staining was detected in slides incubated with control serum and/or goat IgG in the absence of the primary antibody. A Nikon DS-Ri1 epifluorescence microscope was used to acquire digital images using an Eclipse E400 digital camera and NIS-Elements software version 3.0 (Nikon).
Qualitative and quantitative analyses of stained cells were performed using a self-developed plugin for ImageJ (ImageJ 1.43, National Institutes of Health, Bethesda, MD, USA).
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2

Immunofluorescent Profiling of Cx3cr1-GFP Mouse Brain

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Hypothalamic sections from Cx3cr1-GFP mice were incubated with either rabbit anti-Iba1 (1: 1,000; Dako), mouse monoclonal Anti-GFAP-Cy3 (1: 1,000; Sigma), mouse anti-NeuN (1: 1,000; Millipore) or chicken anti-Vimentin (1: 5,000; Abcam). After primary incubation overnight at 4ºC, all wells were processed for 1 h with appropriate fluorescent secondary antibodies (Alexa Fluor 594 donkey anti-rabbit (1: 1,500, Life Technologies); Alexa Fluor 488 donkey anti-chicken (1:500, Jackson Immuno Research); Alexa Fluor 594 goat anti-rabbit (1:500, Invitrogen); Alexa Fluor 594 goat antichicken (1:500, Jackson Immuno Research), Alexa Fluor 594 donkey anti-mouse (1:500, Invitrogen)). Stained sections were imaged using a cooled CCD camera attached to a DS-Ri1 epifluorescence microscope (Nikon, Japan).
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