Fm4 64 dye t 3166

Manufactured by Thermo Fisher Scientific

FM4-64 dye (T-3166) is a fluorescent probe used for labeling cell membranes. It is a lipophilic dye that fluoresces upon binding to the outer leaflet of the plasma membrane. The dye can be used to visualize and track membrane dynamics in live cells.

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Lab products found in correlation

Ezview red anti flag m2 affinity gel, by Merck Group (1 mentions) 3xflag peptide, by Merck Group (1 mentions) Ecl prime western blotting detection reagent, by GE Healthcare (1 mentions) Ezview red anti ha affinity gel, by Merck Group (1 mentions) Phenylmethanesulfonylfluoride p7626, by Merck Group (1 mentions)

Spelling variants of this product

FM4-64 (599 citations) FM4-64 dye (98 citations)

2 protocols using fm4 64 dye t 3166

Affinity Purification of Protein Complexes

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EZview Red ANTI-FLAG M2 Affinity Gel (F2426), EZview Red ANTI-HA Affinity Gel (E6779), 3xFLAG Peptide (F4799), HA Peptide (I2149), Glutathione−Agarose (G4510), N-Ethylmaleimide (S1638), Iodoacetamide (GERPN6302), aprotinin (A1153), pepstatin (P5318) and Phenylmethanesulfonyl fluoride (P7626) were purchased from Sigma-Aldrich (St Louis, MO). K48-, and K63-linked poly-ubiquitin chains (Ub2-7) were purchased from Boston Biochem (Cambridge, MA) and K63-linked tetra-ubiquitin was purchased from UBPBio (Aurora, CO). Protease inhibitor tablet (PI88665), Coomassie Brilliant Blue R-250 Dye (20278), and FM4-64 dye (T-3166) were purchased from ThermoFisher Scientific (San Jose, CA). ECL Prime Western Blotting Detection Reagent (RPN2236) was purchased from GE healthcare Life Sciences (Marlborough, MA).

Xu P., Hankins H.M., MacDonald C., Erlinger S.J., Frazier M.N., Diab N.S., Piper R.C., Jackson L.P., MacGurn J.A, & Graham T.R. (2017). COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal. eLife, 6, e28342.

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Affinity Purification of Deubiquitinases

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ANTI-FLAG M2 Magnetic Beads (M8823), EZview Red ANTI-FLAG M2 Affinity Gel (F2426), 3xFLAG Peptide (F4799), N-ethylmaleimide (E3876), iodoacetamide (GERPN6302), 1,10-phenanthroline (131377), N-ethylmaleimide (E3876), deubiquitinase inhibitor PR-619 (SML0430), protease inhibitor tablets (04693159001), and phosphatase inhibitors tablets (PHOSS-RO) were purchased from MilliporeSigma (St Louis, MO). Coomassie Brilliant Blue R-250 Dye (20278) and FM4-64 dye (T-3166) were purchased from Thermo Fisher Scientific (San Jose, CA). ECL Prime Western Blotting Chemiluminescent Substrate (34580) and Pierce Anti-HA Agarose (26181) were purchased from Thermo Scientific (Rockford, IL). Deubiquitinases (DUBs) Usp2 (E-504), MINDY2 (E-620), MINDY3 (E-621), OTULIN (E-558), AMSH (E-548B), K6-ubiquitin trimer (Ub3) chains (UC-20-025), K11-Ub3 chains (UC-50-025), K29- Ub3 chains (UC-85-025), and K33-Ub3 (UC-105-025) were from BostonBiochem – R&D Systems, Inc (MN, USA).

Date S.S., Xu P., Hepowit N.L., Diab N.S., Best J., Xie B., Du J., Strieter E.R., Jackson L.P., MacGurn J.A, & Graham T.R. (2022). Ubiquitination drives COPI priming and Golgi SNARE localization. eLife, 11, e80911.

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