Tgf β1

Manufactured by GLPBIO

Sourced in China

TGF-β1 is a laboratory reagent used for research purposes. It is a recombinant human transforming growth factor beta 1 protein. TGF-β1 is a multifunctional cytokine that plays a role in cell growth, differentiation, and other cellular processes.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Tgf β1, by Cayman Chemical (1 mentions) Rpmi 1640 medium, by Procell (1 mentions) Mkn 74, by Procell (1 mentions) 6 gingerol, by GLPBIO (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Mrc 5, by Procell (1 mentions)

2 protocols using tgf β1

Fibrosis Modulation with 6-Gingerol and SIRT1

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Human embryonic lung fibroblasts MRC-5 were purchased from Procell Life Science & Technology (Wuhan, China) and cultured in DMEM (Gibco, United States) supplemented with 10% fetal bovine serum (FBS, Invitrogen, CA, United States), 1% penicillin/streptomycin (Invitrogen) in 5% CO 2 atmosphere at 37°C.
The cells were randomly divided into different groups: control, TGF-β1 (Abmole, Shanghai, China), TGF-β1 + 6-gingerol, TGF-β1 + 6-gingerol + EX-527 [Sirtuin1 (SIRT1) inhibitor, purity > 98.00%, GlpBio, United States]. To induce fibrosis, cells were exposed to 10 ng/mL TGF-β1 for 48 h. Other experimental groups were exposed to 10 ng/mL TGF-β1 and 6-gingerol (10 or 20 μM) or EX-527 (10 μM). Untreated cells were used as the control group.

Liu L., Yu N., Leng W., Lu Y., Xia X, & Yuan H. (2022). 6-Gingerol, a functional polyphenol of ginger, reduces pulmonary fibrosis by activating Sirtuin1. Allergologia et immunopathologia, 50(2).

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Regulation of GC and MFC Cell Lines

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Human GC cell lines (AGS and MKN-74) and Mouse Forestomach Carcinoma (MFC) cell line were purchased from the Procell Life Science and Technology (Wuhan, China). These cells were cultured in RPMI 1640 medium (Procell Life Science and Technology, Wuhan, China) with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA). Culture medium was changed every 2 days. Cells were pretreated with SR1078 (RORα activator, GC16392, GlpBio, Montclair, CA, USA), 3PO (3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one, PFKFB3 inhibitor, MCE, USA), DHEA (Dehydroepiandrosterone, G6PD inhibitor, MCE, USA) and glucose for 24 h. Cells were pretreated with TGF-β1 (Transforming growth factor beta 1, Cayman chemical, USA) for 48 h. SR1078 was dissolved in phosphate buffer saline (PBS). 3PO, DHEA, TGF-β1 and fluorouracil (GC14466, GlpBio, Montclair, CA, USA) were dissolved in dimethyl sulfoxide (DMSO).

Wang X., Zhang J., Wu Y., Zhang Y., Zhang S., Li A., Wang J, & Wang Z. (2024). RORα inhibits gastric cancer proliferation through attenuating G6PD and PFKFB3 induced glycolytic activity. Cancer Cell International, 24, 12.

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