Plasma biochemical parameters were measured after overnight fasting including triglycerides, total cholesterol, creatinine, and glucose, which were measured using standard commercial methods on a parallel, multichannel analyzer (Hitachi7170A, Tokyo, Japan) as in our recent report 12 (link). Peripheral complete blood cell count was determined using an automated cell counter (XE-2100 Hematology Alpha Transportation System, Sysmex Corporation, Kobe, Japan). To minimize the confounding effect of infection, subjects with a white blood cell (WBC) count below 4.0 × 109/l or greater than 10.0 × 109/l were re-checked and studied extensively to rule out the existence of chronic infections on the basis of physicians interview, physical examination, and urinalysis.
Xe 2100 hematology alpha transportation system
Manufactured by Sysmex
Sourced in Japan
The XE-2100 Hematology Alpha Transportation System is a laboratory equipment product designed for automated hematology analysis. It is capable of processing blood samples and providing comprehensive data on various blood parameters.
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Lab products found in correlation
6 protocols using xe 2100 hematology alpha transportation system
1
Plasma Biochemical and Hematological Evaluation
2
Comprehensive Peripheral Blood Analysis
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Peripheral blood samples were obtained from the antecubital vein prior to the initiation of any oncological treatment. Peripheral leukocyte analyses were conducted using an automated cell counter (XE-2100 Hematology Alpha Transportation System; Sysmex, Kobe, Japan), and included total leukocyte count, differential neutrophil count, monocyte count, lymphocyte count, and red blood cell (RBC) parameters (hemoglobin, hematocrit, mean corpuscular hemoglobin, mean corpuscular-hemoglobin concentration (MCHC)), platelet count, red cell distribution width-standard deviation (RDW-SD), and RDW-coefficient of variation (RDW-CV). Absolute counts of leukocyte subtypes were calculated as the product of its percentage and total leukocyte count.
3
Comprehensive Health Screening Protocol
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After fasting for at least 8 hours, a peripheral blood sample was taken from the worker’s antecubital vein. Complete blood count and serum uric acid, aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose, HbA1c, and lipid panel (including plasma total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglycerides) were also analyzed during the health check, and were determined in all workers using standard commercial methods with a parallel, multichannel analyzer (Hitachi 7170A, Tokyo, Japan) as described in our previous reports [20 (link),21 (link)]. Peripheral leukocyte count was analyzed with an automated cell counter (XE-2100 Hematology Alpha Transportation System, Sysmex Corporation, Kobe, Japan). The TyG index was calculated as ln(fasting triglycerides (mg/dL) × fasting plasma glucose (mg/dL)/2) [22 (link)].
4
Comprehensive Cardiometabolic Biomarker Panel
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Levels of total cholesterol, LDL-C, complete blood cell count, serum triglycerides, HDL-C, albumin, blood urine nitrogen (BUN), uric acid, and glucose were measured from fasting blood samples 30 (link). The Jaffe method was used to calculate serum creatinine. Hemoglobin A1c was measured using an automated analyzer (HLC-723G8, Tosoh Corp., Tokyo, Japan). CK-MB and troponin I serum levels were measured using chemiluminescent microparticle immunoassays. Total leukocyte count and the lymphocyte, neutrophil and monocytes proportions were measured using an automated cell counter (XE-2100 Hematology Alpha Transportation System; Sysmex, Kobe, Japan). Absolute leukocyte subtype counts were calculated as the product of its proportion and total leukocyte count. The CKD-EPI formula was utilized to determine estimated glomerular filtration rate (eGFR) 31 (link). An immunochemistry system (Beckman Coulter IMMAGE) was used to evaluate plasma levels of high-sensitivity C-reactive protein (hs-CRP). The detection limit was 0.2 mg/L, and all measurements were made twice.
5
Metabolic Profiling of Occupational Exposure
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Peripheral blood samples were taken from the antecubital vein of the workers after fasting for at least 8 h. Complete blood cell count and levels of serum glucose, HbA1c, uric acid, and lipid profiles [including plasma triglycerides, total cholesterol, low-density lipoprotein cholesterol (LDL-C), and HDL-C] were also measured during the health checkups and were determined in all workers using standard commercial methods with a parallel, multichannel analyzer (Hitachi 7170A, Tokyo, Japan) as described in our previous report [19 (link), 20 (link)]. Peripheral leukocyte analysis was performed using an automated cell counter (XE-2100 Hematology Alpha Transportation System, Sysmex Corporation, Kobe, Japan). The concentrations of plasma resistin were determined using commercial enzyme immunoassay kits (Phoenix Pharmaceuticals, Belmont, CA). The intraassay coefficient of variation was 2.1–5.2% for resistin. Samples were measured in duplicate in a single experiment.
6
Fasting Blood Biomarker Evaluation
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On the morning of the procedure and after fasting for at least 8 hours, blood samples were collected, stored, and analyzed by one single laboratory. Serum levels of triglycerides, total cholesterol, low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol, and glucose were measured using standard commercial methods on a parallel, multichannel analyzer (Hitachi 7170A) as in our previous reports.17 Total leukocyte count, RBC, hemoglobin, hematocrit, MCV, and MCH were measured using an automatic hematological analyzer (XE‐2100 Hematology Alpha Transportation System; Sysmex Corporation). To minimize the confounding effect of infection, subjects with a WBC count below 4.0 × 109/L or greater than 10.0 × 109/L were rechecked and examined extensively for possible occult or chronic infections. Any specimen with abnormal or atypical leukocytes were re‐analyzed and excluded.
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