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A1r confocal laser microscope

Manufactured by Fortrea
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The A1R Confocal Laser Microscope is a high-performance imaging system designed for advanced microscopy applications. It utilizes a laser light source and a confocal optical system to capture detailed, high-resolution images of samples. The microscope features a variety of technical specifications and capabilities, enabling users to conduct precise and focused observations.

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Lab products found in correlation

Vinculin, by Merck Group (2 mentions) Pannoramic digital slide scanner, by 3DHISTECH (2 mentions) Rm 9106 s1, by Cell Signaling Technology (2 mentions) Phospho s6, by Cell Signaling Technology (2 mentions) Phospho met, by Cell Signaling Technology (2 mentions) Phospho vegfr2, by Cell Signaling Technology (2 mentions) Phospho erk1 2, by Cell Signaling Technology (2 mentions) Phospho mtor, by Cell Signaling Technology (2 mentions) Phospho akt, by Cell Signaling Technology (2 mentions) Phospho p70s6k, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Ripa buffer, by Boston BioProducts (2 mentions)

2 protocols using a1r confocal laser microscope

1

Immunohistochemical and Western Blot Analysis of Biological Samples

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were fixed in 10% formalin overnight and embedded in paraffin. Immunohistochemical (IHC) and immunofluorescence (IF) staining was performed as previously described11 (link),14 (link). IHC slides were scanned with Pannoramic Digital Slide Scanner (3DHISTECH) and images were cropped from virtual slides in Pannoramic Viewer. IF slides were imaged with Nikon A1R Confocal Laser Microscope and quantified with ImageJ. Primary antibodies used include CK5 (Covance, PRB-160P), CK8 (Covance, MMS-162P), Ki67 (Fisher, RM-9106-S1), cleaved caspase 3 (Cell Signaling Technology, 9661), Gr-1 (BioLegend, 108401), phospho-S6 (Cell Signaling Technology, 4858). For Western blot analysis, cells or fresh tissues were lysed on ice using RIPA buffer (Boston BioProducts) supplemented with protease and phosphatase inhibitors (Roche). Western blot procedure was performed as previously described11 (link),14 (link). Primary antibodies used include phospho-Met (Cell Signaling Technology, 3077), phospho-VEGFR2 (Cell Signaling Technology, 3770), phospho-Erk1/2 (Cell Signaling Technology, 4370), phospho-Akt (Cell Signaling Technology, 4060), phospho-mTOR (Cell Signaling Technology, 5536), phospho-p70 S6K (Cell Signaling Technology, 9234), phospho-S6 (Cell Signaling Technology, 4856), and vinculin (Millipore, 05-386).
Lu X., Horner J.W., Paul E., Shang X., Troncoso P., Deng P., Jiang S., Chang Q., Spring D.J., Sharma P., Zebala J.A., Maeda D.Y., Wang Y.A, & DePinho R.A. (2017). Effective Combinatorial Immunotherapy for Castration Resistant Prostate Cancer. Nature, 543(7647), 728-732.
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2

Immunohistochemical and Western Blot Analysis of Biological Samples

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were fixed in 10% formalin overnight and embedded in paraffin. Immunohistochemical (IHC) and immunofluorescence (IF) staining was performed as previously described11 (link),14 (link). IHC slides were scanned with Pannoramic Digital Slide Scanner (3DHISTECH) and images were cropped from virtual slides in Pannoramic Viewer. IF slides were imaged with Nikon A1R Confocal Laser Microscope and quantified with ImageJ. Primary antibodies used include CK5 (Covance, PRB-160P), CK8 (Covance, MMS-162P), Ki67 (Fisher, RM-9106-S1), cleaved caspase 3 (Cell Signaling Technology, 9661), Gr-1 (BioLegend, 108401), phospho-S6 (Cell Signaling Technology, 4858). For Western blot analysis, cells or fresh tissues were lysed on ice using RIPA buffer (Boston BioProducts) supplemented with protease and phosphatase inhibitors (Roche). Western blot procedure was performed as previously described11 (link),14 (link). Primary antibodies used include phospho-Met (Cell Signaling Technology, 3077), phospho-VEGFR2 (Cell Signaling Technology, 3770), phospho-Erk1/2 (Cell Signaling Technology, 4370), phospho-Akt (Cell Signaling Technology, 4060), phospho-mTOR (Cell Signaling Technology, 5536), phospho-p70 S6K (Cell Signaling Technology, 9234), phospho-S6 (Cell Signaling Technology, 4856), and vinculin (Millipore, 05-386).
Lu X., Horner J.W., Paul E., Shang X., Troncoso P., Deng P., Jiang S., Chang Q., Spring D.J., Sharma P., Zebala J.A., Maeda D.Y., Wang Y.A, & DePinho R.A. (2017). Effective Combinatorial Immunotherapy for Castration Resistant Prostate Cancer. Nature, 543(7647), 728-732.
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