Nicotinamide adenine dinucleotide

Bovine serum albumin and bleomycin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Trichloroacetic acid was acquired from Merck (Mumbai, India), and thiobarbituric acid (TBA) and nitroblue tetrazolium chloride were purchased from Loba Chemie Pvt. Ltd., (Mumbai, India). 5,5′-Dithiobis(−2-nitro benzoic acid), phenazonium methosulfate, nicotinamide adenine dinucleotide and reduced glutathione (GSH) were purchased from Sisco Research Laboratories Pvt., Ltd., (Mumbai, India). All other chemicals and reagents used were of the highest analytical grade.

2′,7′ − Dichlorofluorescin Diacetate (DCFDA), Vanadium chloride (III), Prednisolone (purity 99%), cetyltrimethylammonium bromide (CTAB), Xylenol orange and Trolox were purchased from Sigma − Aldrich (Burlington, USA). Bovine serum (BSA), o − dianisidine dihydrochloride (ODD), methionine, reduced Glutathione (GSH), oxidised Glutathione, nicotinamide adenine dinucleotide (NADH), hydroxylamine hydrochloride, 2,4 − dinitrophenylhydrazine and riboflavin were purchased from Sisco Research Laboratory (Mumbai, India). Sodium azide, 5, 5 − Dithiobis − 2 − Nitrobenzoic acid (DTNB) and 1 − chloro − 2,4 − di nitro benzene (CDNB) were purchased from Himedia (Maharashtra, India).

Penicilliumchrysogenum SNP5 (MTCC13144) strain was locally isolated from grease-contaminated soil of the diesel loco shed and identified by Microbial Type Culture Collection and gene bank Chandigarh, India^{23 (link)}. Triton X-100, Phenylmethylsulfonyl fluoride (PMSF), Lauryl dimethylamine oxide (LDAO), Nicotinamide adenine dinucleotide (NAD) + hydrogen (NADH), 1, 6- diphenyl- 1, 3, 5- hexatriene (DPH), Acrylamide, Bis Acrylamide, Coomassie brilliant blue R-250, Glycine, Bromophenol blue, Methanol, para-nitrophenol, 1-Bromooctane, Dimethyl Sulphoxide (DMSO), Petroleum ether, diethyl ether, other medium components, and Czapek-dox medium were procured from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Hexadecane and THB (Tetrahydrobiopterin), Acetic acid, and Digitonin were procured from TCI Chemicals Pvt. Ltd. (India). Phosphatidylcholine and cholesterol were purchased from Sigma-Aldrich Chemicals Pvt. Ltd. PageRuler™ Plus Prestained Protein Ladder was procured from Thermo Scientific.