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1 2 dioleoyl sn glycero 3 phosphoserine

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1,2-dioleoyl-sn-glycero-3-phosphoserine is a phospholipid compound used in various research applications. It is a naturally occurring lipid that serves as a structural component in biological membranes. This product can be used as a reagent in cell culture, liposome formulation, and other biochemical studies.

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Lab products found in correlation

1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (5 mentions) Thapsigargin, by Thermo Fisher Scientific (3 mentions) Thapsigargin, by Avanti Polar Lipids (3 mentions) Oxo m, by Merck Group (2 mentions) Dgk inhibitor, by Bio-Techne (2 mentions) 1 2 dioleoyl 9 10 3h rac glycerol, by American Radiolabeled Chemicals (2 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions) 1 2 dioleoyl sn glycerol, by Avanti Polar Lipids (2 mentions) Proteinase k, by Merck Group (2 mentions) Ionomycin, by Merck Group (2 mentions) Oxo m, by Avanti Polar Lipids (2 mentions) Ionomycin, by Avanti Polar Lipids (2 mentions) Dgk inhibitor, by Avanti Polar Lipids (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Phosphatidylserine, by Avanti Polar Lipids (1 mentions) Phosphatidylethanolamine, by Avanti Polar Lipids (1 mentions) 1 2 dioleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine dmpc, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphoethanolamine, by Avanti Polar Lipids (1 mentions) Aβ1 42, by Bachem (1 mentions)

9 protocols using 1 2 dioleoyl sn glycero 3 phosphoserine

1

Purification of Coagulation Factors

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Protein S-free C4BP was purified (29) and APC was activated from in house purified protein C as described (30) (31) (32) . Recombinant FV R506Q/R679Q was expressed and quantified as described (33) , bovine FX (34) and human prothrombin were purified from plasma (35) .
Human α-thrombin was prepared from purified prothrombin (36) . Phospholipids were purchased from Avanti Polar Lipids and include the natural phospholipids phosphatidylserine (PS; brain extract), phosphatidylethanolamine (PE; egg extract) and phosphatidylcholine (PC; egg extract) and the synthetic phospholipids 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-Dioleoyl-sn-glycero-3-phosphoserine (DOPS), and 1,2-Dioleoyl-sn-glycero-3phosphoethanolamine (DOPE). Phospholipid vesicles were prepared by extrusion technique (37) .
Somajo S., Ahnström J., Fernandez-Recio J., Gierula M., Villoutreix B.O, & Dahlbäck B. (2015). Amino acid residues in the laminin G domains of protein S involved in tissue factor pathway inhibitor interaction. Thrombosis and haemostasis, 113(5).
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2

Preparation of Phospholipid Membrane Vesicles

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Ultrapure water (resistivity: 18.2 M cm) was obtained from an ELGA purelab option Q7 system (VEOLIA WATER STI, France). 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium chloride (NaCl) and calcium chloride (CaCl2) were purchased from Sigma (Saint Quentin Fallavier, France). Ethylenediaminetetraacetic acid (EDTA) was from Chimie Plus Laboratoire (Denice, France). 1,2-Dioleoyl-sn-glycero- 3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero- 3-phosphoserine (DOPS), 1,2-dimyristoyl-sn-glycero- 3-phosphocholine (DMPC) and 1,2-dimyristoyl-sn-glycero- 3-phosphoserine (DMPS) were purchased from Avanti Polar Lipids (Coger, France). The phospholipid solutions were prepared by mixing the desired amount of DMPS/DMPC or DOPS/DOPC at 3:1 or 1:3 molar ratio dissolved in chloroform solution at a concentration of 1 mg/mL.
Rebaud S., Simon A., Wang C.K., Mason L., Blum L., Hofmann A, & Girard-Egrot A. (2014). Comparison of VILIP-1 and VILIP-3 Binding to Phospholipid Monolayers. PLoS ONE, 9(4), e93948.
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3

Amyloid-beta Lipid Interactions

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Aβ1–42 was purchased from Bachem and Anaspec (Fremont, CA).
The phospholipids 1.2‐dioleoyl‐sn‐glycero‐3‐phosphoserine (DOPS) and 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphoethanolamine (POPE) were purchased from Avanti Polar Lipids (Alabaster, AL). All other chemicals were purchased from Sigma‐Aldrich (St. Louis, MO).
Martinez Hernandez A., Urbanke H., Gillman A.L., Lee J., Ryazanov S., Agbemenyah H.Y., Benito E., Jain G., Kaurani L., Grigorian G., Leonov A., Rezaei‐Ghaleh N., Wilken P., Arce F.T., Wagner J., Fuhrman M., Caruana M., Camilleri A., Vassallo N., Zweckstetter M., Benz R., Giese A., Schneider A., Korte M., Lal R., Griesinger C., Eichele G, & Fischer A. (2017). The diphenylpyrazole compound anle138b blocks Aβ channels and rescues disease phenotypes in a mouse model for amyloid pathology. EMBO Molecular Medicine, 10(1), 32-47.
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4

Lipid Reagents for Cell Signaling

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Primary and secondary antibodies used in this study are listed in Supplementary Table 1. Oxo-M, Atropine and Ionomycin (Sigma-Aldrich). Thapsigargin (Invitrogen/Life-technologies). DGK inhibitor (R 59–022, Tocris Bioscience). Proteinase K (Sigma-Aldrich). Following concentration of chemicals are used in all the experiments unless noted: Oxo-M, 10μM; Atropine, 50μM; Ionomycin, 2μM; Thapsigargin, 2μM; DGK inhibitor, 50μM. All non radiolabeled lipids were obtained from Avanti Polar Lipids; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 850457; 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 840035; L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), 840046; (NBD)-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 810144; 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] (DGS-NTA(Ni)), 790404; 1-2-dioleoyl-sn-glycerol (DAG), 800811. Radiolabeled lipids were purchased from American Radiolabeled Chemicals (St. Louis, MO); 1,2-Dioleoyl [9,10-3H] rac-glycerol, ART 2185.
Saheki Y., Bian X., Schauder C.M., Sawaki Y., Surma M.A., Klose C., Pincet F., Reinisch K.M, & De Camilli P. (2016). Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nature cell biology, 18(5), 504-515.
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5

Phospholipid Vesicle Preparation and Extrusion

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The phospholipids (Avanti Polar Lipids) 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were mixed in a molar ratio of 60:20:20 and extruded as previously described (46 (link)).
Santamaria S., Reglińska-Matveyev N., Gierula M., Camire R.M., Crawley J.T., Lane D.A, & Ahnström J. (2017). Factor V has an anticoagulant cofactor activity that targets the early phase of coagulation. The Journal of Biological Chemistry, 292(22), 9335-9344.
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6

Quantitative Lipid Composition Analysis

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Sphingomyelin (egg), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS) were purchased from Avanti Polar Lipids and Cholesterol (Chol) was obtained from Northern Lipids. Lipid concentrations were quantified by phosphorus analysis (Rouser et al. 1966) (link). Cholesterol concentration was quantified using the Cholesterol Kit (BioSystems).
Elderdfi M., Zegarlińska J., Jones W, & Sikorski A.F. (2017). MPP1 interacts with DOPC/SM/Cholesterol in an artificial membrane system using Langmuir-Blodgett monolayer. General physiology and biophysics, 36(4).
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7

Characterization of Phospholipid Reagents

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l-α-Phosphatidylcholine (egg, chicken; ePC), DOPC, 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), PI4P, PI, and NBD-PS were purchased from Avanti Polar lipids (Alabaster, AL). ATP, adenylyl-imidodiphosphate (AMP-PNP), and octyl-β-d-glucopyranose (OGP) were purchased from Sigma-Aldrich, sodium hydrosulfite (sodium dithionite) from Fischer Scientific, CHAPS from Anatrace (Maumee, OH), Protease Arrest from G-Biosciences (St. Louis, MO), and 1D4 peptide from Celtek Peptides (Franklin, TN). HEK293T and rat PC12 cells were purchased from ATCC. Rho 1D4 antibody used for preparation of immunoaffinity columns was generated in-house (Hodges et al., 1988 (link); Quazi and Molday, 2013 (link)) and purchased from UBC through Flintbox (www.rho1d4.com/); primary antibodies against calnexin, actin, and tubulin were from Abcam; fluorescent-tagged secondary antibodies for immunofluorescence imaging were from Molecular Probes; and anti-Cdc50-7F4 (CDC50A) primary antibodies used in Western blots and immunofluorescence analysis were raised in-house (Coleman and Molday, 2011 (link)). Restriction enzymes, T4-DNA ligase, Antarctic Phosphatase, and CaMKII kit were procured from New England Biolabs, DNA polymerase was from Thermo Scientific, and the QuikChange Site-Directed Mutagenesis Kit was from Agilent Technologies.
Chalat M., Moleschi K, & Molday R.S. (2017). C-terminus of the P4-ATPase ATP8A2 functions in protein folding and regulation of phospholipid flippase activity. Molecular Biology of the Cell, 28(3), 452-462.
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8

Lipid Reagents for Cell Signaling

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Primary and secondary antibodies used in this study are listed in Supplementary Table 1. Oxo-M, Atropine and Ionomycin (Sigma-Aldrich). Thapsigargin (Invitrogen/Life-technologies). DGK inhibitor (R 59–022, Tocris Bioscience). Proteinase K (Sigma-Aldrich). Following concentration of chemicals are used in all the experiments unless noted: Oxo-M, 10μM; Atropine, 50μM; Ionomycin, 2μM; Thapsigargin, 2μM; DGK inhibitor, 50μM. All non radiolabeled lipids were obtained from Avanti Polar Lipids; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 850457; 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 840035; L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), 840046; (NBD)-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 810144; 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] (DGS-NTA(Ni)), 790404; 1-2-dioleoyl-sn-glycerol (DAG), 800811. Radiolabeled lipids were purchased from American Radiolabeled Chemicals (St. Louis, MO); 1,2-Dioleoyl [9,10-3H] rac-glycerol, ART 2185.
Saheki Y., Bian X., Schauder C.M., Sawaki Y., Surma M.A., Klose C., Pincet F., Reinisch K.M, & De Camilli P. (2016). Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nature cell biology, 18(5), 504-515.
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9

Antibody-Based Protein Detection Protocol

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6x-His Tag monoclonal primary antibody (His.H8, ThermoFisher), STIM1 monoclonal antibody (CDN3H4, ThermoFisher), mCherry polyclonal antibody (PA5-34974, ThermoFisher), GAPDH polyclonal antibody (G9545, SigmaAldrich), goat anti-mouse and anti-rabbit secondary antibodies DyLight 800 and DyLight 680 (ThermoFisher) were used for western blots. OxotremorineM (Sigma-Aldrich), thapsigargin (Invitrogen/Life-technologies), isopropylthio-β-galactoside (ThermoFisher), Fura-PE3 AM (Teflabs), Protease Inhibitor (PI) Cocktail Set 1 (Calbiochem/EMD Millipore), 0.01% poly-L-lysine solution (Sigma), Ni-IDA resin (Protino/Macherey-Nagel), Lipofectamine2000 (ThermoFisher). Following concentration of chemicals are used in all the experiments unless noted: OxotremorineM, 10 μM; thapsigargin, 1 μM; isopropylthio-β-galactoside, 0.5 μM. All lipids were obtained from Avanti Polar Lipids; 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 850375C; 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine 16:0-18:1 (POPS), 840034C; 1,2-dioleoyl-sn-glycero-3-phosphoserine (DOPS), 840035; L-α-phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), 840046; L-a-phosphatidylinositol-4-phosphate (PI(4)P), 840045X; L-a-phosphatidylinositol (PI), 84042C.
Besprozvannaya M., Dickson E., Li H., Ginburg K.S., Bers D.M., Auwerx J, & Nunnari J. (2018). GRAM domain proteins specialize functionally distinct ER-PM contact sites in human cells. eLife, 7, e31019.
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