E faecium atcc 700221

Manufactured by American Type Culture Collection

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E. faecium ATCC 700221 is a strain of the bacterium Enterococcus faecium, obtained from the American Type Culture Collection (ATCC). This bacterial strain is available for research purposes.

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Lab products found in correlation

E faecalis atcc 29212, by American Type Culture Collection (2 mentions) S aureus atcc 29213, by American Type Culture Collection (2 mentions) Bhi media, by BD (1 mentions) Fm4 64, by Thermo Fisher Scientific (1 mentions) E coli atcc 25922, by American Type Culture Collection (1 mentions) S epidermidis atcc 12228, by American Type Culture Collection (1 mentions) C albicans atcc 90028, by American Type Culture Collection (1 mentions) Candida krusei atcc 6258, by American Type Culture Collection (1 mentions) Candida parapsilosis atcc 22019, by American Type Culture Collection (1 mentions)

3 protocols using e faecium atcc 700221

Culturing Enterococcus faecium

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E. faecium ATCC 700221 obtained from the American Type Culture Collection (Manassas, VA, USA) was used in this study. Bacteria were grown in brain heart infusion (BHI) media (BD Biosciences, San Jose, CA, USA) at 37°C.

Kim M.H., Kim S.Y., Son J.H., Kim S.I., Lee H., Kim S., Shin M, & Lee J.C. (2019). Production of Membrane Vesicles by Enterococcus faecium Cultured With or Without Subinhibitory Concentrations of Antibiotics and Their Pathological Effects on Epithelial Cells. Frontiers in Cellular and Infection Microbiology, 9, 295.

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Antimicrobial Susceptibility Assays

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The bacterial strains S. aureus ATCC 29213, S. aureus ATCC 29247, S. aureus ATCC BAA-41, E. faecium ATCC 49624, E. faecium ATCC 700221, E. faecalis ATCC 29212, S. epidermidis ATCC 12228, E. coli ATCC 25922, and K. pneumoniae ATCC BAA-1144 used in the antimicrobial susceptibility assays were purchased from American Type Culture Collection (ATCC, USA). B. subtilis strain 168 was already available in our in-house collection. E. coli JM109 WM647 was kindly provided by Dr. W. Margolin (University of Texas-Houston Medical School). FM 4–64 was purchased from Invitrogen (Eugene, Oregon). All other chemicals and reagents were purchased from Sigma-Aldrich. Stock solutions were prepared in DMSO. The final percentage of DMSO in the assays was 1% (v/v) for all experiments.

Sun N., Chan F.Y., Lu Y.J., Neves M.A., Lui H.K., Wang Y., Chow K.Y., Chan K.F., Yan S.C., Leung Y.C., Abagyan R., Chan T.H, & Wong K.Y. (2014). Rational Design of Berberine-Based FtsZ Inhibitors with Broad-Spectrum Antibacterial Activity. PLoS ONE, 9(5), e97514.

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Antimicrobial Susceptibility Testing Strains

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S. aureus ATCC 29213, C. albicans ATCC 90028, E. faecalis ATCC 29212, E. faecium ATCC 700221, Candida krusei ATCC
6258, Candida parapsilosis ATCC 22019, Candida tropicalis ATCC 13803, Candida
glabrata ATCC MYA-2950, and A. fumigatus ATCC MYA-3626 were obtained from the American Type Culture Collection
(Manassas, VA). E. coli BW25113 (wild-type)
and JW5503-1 (tolC derivative of BW25113) were obtained
from the Coli Genetic Stock Center (New Haven, CT). S. aureus #1180973 (MRSA/MDR), A.
baumannii #1186154, C. albicans #1186858 (azole resistant), and C. albicans #1181444 (echinocandin resistant) were obtained from the JMI Laboratories
SENTRY strain collection.^{41 (link)}P. aeruginosa (ΔmexAB-oprM::Cm ΔmexCD-oprJ::Gm ΔmexEF-oprN::ΩHg) PAM1626 is a derivative of PAO1 and was
the kind gift of O. Lomovskaya.^{36 (link)}

Martínez-Fructuoso L., Arends S.J., Freire V.F., Evans J.R., DeVries S., Peyser B.D., Akee R.K., Thornburg C.C., Kumar R., Ensel S., Morgan G.M., McConachie G.D., Veeder N., Duncan L.R., Grkovic T, & O’Keefe B.R. (2023). Screen for New Antimicrobial Natural Products from the NCI Program for Natural Product Discovery Prefractionated Extract Library. ACS Infectious Diseases, 9(6), 1245-1256.

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