Tannerella forsythia atcc 43037

Tannerella forsythia ATCC 43037 (American Type Culture Collection – ATCC, Manassas, VA, United States) (Tanner et al., 1986 (link); Friedrich et al., 2015 (link)) and its mutants (Table 1) were grown anaerobically in brain heart infusion (BHI) media (Oxoid, Basingstoke, United Kingdom), supplemented with yeast extract (Sigma-Aldrich, Vienna, Austria), L-cysteine (Sigma-Aldrich), hemin (Sigma-Aldrich), menadione (Sigma-Aldrich), N-acetylmuramic acid (Carbosynth, Compton, United Kingdom), and horse serum (Thermo Fisher Scientific, Vienna, Austria) as described previously (Tomek et al., 2017 (link)). Media were supplemented with 50 μg/ml gentamicin, 5 μg/ml Erm or 10 μg/ml Cat, when appropriate.
Escherichia coli strains (Table 1) were grown under standard conditions in LB medium supplemented with 100 μg/ml ampicillin, when appropriate.

The strains Tannerella forsythia ATCC 43037 and Prevotella intermedia ATCC 25611 were acquired from commercial providers (American Type Culture Collection, Manassas, USA), and cultivated on COL agar plates (BD, Franklin Lakes, USA) and in PYG modified with 5 μg/ml hemin and 1% vitamin K₁ under anaerobic atmosphere (37 °C, 80% N₂/10% H₂/10% CO₂). Growth behavior in cell culture medium DMEM with 10% FCS was validated via optical density at 600 nm in parallel with PYG and BHI and parallel CFU determination of BHI probes on BHI agar plates over 24 h under anaerobic conditions.

Tannerella forsythia ATCC 43037 and Porphyromonas gingivalis W83 were obtained from the American Type Culture Collection (Manassas, VA, United States). Cultivation was done anaerobically at 37 °C in brain heart infusion medium (37 g l^− 1; Oxoid, Basingstoke, United Kingdom), supplemented with yeast extract (10 g l^− 1; Sigma, Vienna, Austria), L-cysteine (1 g l^− 1; Sigma), hemin (5 mg ml^− 1; Sigma) and menadione (2 mg ml^− 1; Sigma). For T. forsythia, 5% (v/v) horse serum (Thermo Fisher Scientific, Vienna, Austria) and MurNAc (20 μg ml^− 1; Carbosynth, Compton, United Kingdom) were added.
Escherichia coli DH5α was cultivated in Luria Bertani broth (Miller’s LB broth base; Thermo Fisher Scientific) at 37 °C with shaking.