Sw579

Human thyroid cancer cell line TT, TPC1 and SW579 were purchased from American Type Culture Collection (USA). After thawing, TT cells were cultured in F12 K (N3520; Sigma, USA) medium supplemented with 10 % fetal bovine serum (Gibco, USA) and NaHCO₃ 2.5 g/L at 37 °C in a humidified atmosphere containing 5 % CO₂. TPC1 cells were cultured in DMEM medium supplemented with 10 % fetal bovine serum at 37 °C in a humidified atmosphere containing 5 % CO₂. SW579 cells were cultured in L15 medium supplemented with 10 % fetal bovine serum at 37 °C in a humidified atmosphere without CO₂. After three passages, cells were used for viral infection.

Normal human thyroid follicular epithelial cell Nthy-ori3-1 cell and human thyroid cancer K-1, TPC-1, SW579, FTC133 and XTC-1 purchased from American Type Culture Collection (ATCC) were cultured in RPMI-1640 medium with 10 % fetal bovine serum (FBS), 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, and 1 % penicillin-streptomycin (Sigma, USA) in the humidified incubator with 5 % CO₂ at 37 °C. Cells were grown in monolayer and conventionally passaged when cell attachment rate reached 90 %.
The PANDAR-siRNA expression vector was constructed based on the full length of wild-type lncRNA-PANDAR coding sequence by Genechem Biotech (Shanghai, China). The target sequence for siRNA- PANDAR vector construction was antisense: PANDAR-1: 5'-GCAATCTACAACCTGTC TT3', PANDAR-1:5'TTTCGAACGGAAC AGAGACUUAUACAGATT-3'. SiRNA vector with no silenced PANDAR sequence (si-NC) was transfected into thyroid cancer cells as the controls. Cells were plated and cultured in growth media until cell density reached 70 % prior to siRNA transfection and cell transfections were conducted with Lipofectamine 2000 reagent based on the manufacturer's protocol (Invitrogen, USA). Cells were harvested after 48 h for qRT-PCR and Western blot analyses.

Human papillary thyroid cancer cell line K1 and TPC-1, human thyroid squamous cell carcinoma cell line SW579 and human thyroid cell line Nthy-ori 3–1 purchased from American Type Culture Collection (ATCC) were cultured in DMEM medium with 10% fetal bovine serum (FBS), and incubated in incubator with 5% CO₂ at 37°C. Cells shown monolayer growth and cell passage was carried out when the cell attachment rate reach 90%. Cells were conventionally passaged, and the primary medium was moved. Cells were washed two times with PBS and digested in 0.25% trypsin, and the 0.25% trypsin was removed after increased intercellular space was observed. Finally, cells were made into single cell suspension using RPMI-1640 medium containing calf serum with a volume fraction of 10%.