L glutamine

Manufactured by BBI Lifesciences

L-glutamine is a non-essential amino acid that plays a crucial role in various biological processes. It serves as a building block for proteins and is involved in the synthesis of other amino acids. L-glutamine is an important substrate for cellular energy production and is also involved in the regulation of acid-base balance in the body.

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Lab products found in correlation

Geneticin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Bovogen (1 mentions) Penicillin streptomycin, by BBI Lifesciences (1 mentions) Matrigel matrix, by Corning (1 mentions) L buthionine sulfoximine bso, by Merck Group (1 mentions) Clm 1396 5, by Cambridge Isotopes (1 mentions) D glucose, by Cambridge Isotopes (1 mentions)

2 protocols using l glutamine

Generating Prion Protein Mutant Expressing Cell Lines

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RK13 rabbit epithelial cells (American Type Culture Collection CCL-37) were transfected with mammalian expression vector pcDNA3.1(+)-moPrPWT to express the full-length PrP^C. A separate set of RK13 cells was transfected with pcDNA3.1(+)-moMet4-1 or pcDNA3.1(+)-moΔN6 to express mutant PrP Met4-1 and ΔN6, respectively. These expression vectors were constructed by Generay Biotechnology. All plasmids were linearized by digestion with ScaI (Thermo Fisher Scientific; catalog number: FD0434) before transfection. All transfected cells were selected with 600 to 1200 μg/ml of Geneticin (Gibco; catalog number: 11811023) in Dulbecco's modified Eagle's medium (Gibco; catalog number: 11965092) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Bovogen; catalog number: SFBS), 1% (v/v) l-glutamine (BBI Life Sciences), and 1% (v/v) penicillin/streptomycin (BBI Life Sciences; catalog number: E607011) as described previously (54 (link)).

Zhang X., Pan Y.H., Chen Y., Pan C., Ma J., Yuan C., Yu G, & Ma J. (2021). The protease-sensitive N-terminal polybasic region of prion protein modulates its conversion to the pathogenic prion conformer. The Journal of Biological Chemistry, 297(5), 101344.

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Murine FGF-basic Proliferation Assay

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Recombinant mouse FGF-basic (FGFb, PMG0034) was from Gibco by life technologies. D-Luciferin (S7763) was from Selleck.cn. EGF (E9644), insulin (I3536), BSA (V900933), DMEM-high glucose (D0422-100ML), GSH-MEE (#G1404), vitamin E analog (a-tocopherol; #T3251) and L-buthionine-sulfoximine (BSO; B2515) were from Sigma-Aldrich. Matrigel Matrix (354262) was from Corning. B27 (1639356) was from Gibico. L-Glutamic acid (D316BA0011) was from BBI Life Sciences. L-Glutamine ( 13 C 5 , 99%; CLM-1822-H-0.5), L-Glutamic acid ( 13 C 5 , 99%; CLM-1800-H-1), [ 13 C 6 , 15 N 2 ]-cystine (CNLM-4244-H-PK) and D-glucose ( 13 C 6 , 99%; CLM-1396-5) were from Cambridge Isotope Laboratories. Annexin V-FITC reagent (C1062S), ROS Kit (S0033) was from Beyotime.

Guo W., Li K., Sun B., Xu D., Tong L., Yin H., Liao Y., Song H., Wang T., Jing B., Hu M., Liu S., Kuang Y., Ling J., Li Q., Wu Y., Wang Q., Yao F., Zhou B.P., Lin S.H, & Deng J. (2021). Dysregulated Glutamate Transporter SLC1A1 Propels Cystine Uptake via Xc^- for Glutathione Synthesis in Lung Cancer. Cancer research, 81(3).

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