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Slanetz and bartley agar medium

Manufactured by Biolife
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Sourced in Italy

Slanetz and Bartley agar medium is a selective culture medium used for the detection and enumeration of Enterococcus species. It is primarily used in the microbiological analysis of water, food, and environmental samples.

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Lab products found in correlation

Cellulose ester membrane, by Merck Group (2 mentions)

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Slanetz Bartley Agar (3 citations)

3 protocols using slanetz and bartley agar medium

1

Enterococci Quantification in Water Samples

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A 100-mL aliquot of each sample was filtered through a cellulose ester membrane (47 mm Ø and 0.45-µm pore size; Millipore, Milan, Italy). The membrane was placed over a Slanetz and Bartley agar medium (Biolife Italiana srl, Milan, Italy) and incubated at 36 ± 1 °C for 48 h. The colonies ranged in color from pink to dark red and brown, but only catalase- and esculin hydrolysis-positive colonies were considered to be enterococci [57 ]. The results were reported as CFU/100 mL.
Montagna M.T., De Giglio O., Calia C., Pousis C., Triggiano F., Murgolo S., De Ceglie C., Bagordo F., Apollonio F., Diella G., Narracci M., Acquaviva M.I., Ferraro G.B., Mancini P., Veneri C., Brigida S., Grassi T., De Donno A., Di Iaconi C., Caputo M.C., Cavallo R.A., La Rosa G, & Mascolo G. (2020). Microbiological and Chemical Assessment of Wastewater Discharged by Infiltration Trenches in Fractured and Karstified Limestone (SCA.Re.S. Project 2019–2020). Pathogens, 9(12), 1010.
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2

Enumeration of Enterococci in Water

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A 100-ml aliquot of each water sample was filtered through a cellulose ester membrane (47 mm Ø and 0.45 μm pore size; Millipore, Milan, Italy). The membrane was placed over a Slanetz and Bartley agar medium (Biolife Italiana srl, Milan, Italy) and incubated at 36 ± 1 °C for 48 h. The colonies ranged in color from pink to dark red and brown, but only catalase and esculin hydrolysis positive ones were considered to be ENT (EN ISO 7899-2: 2003 ).
De Giglio O., Barbuti G., Trerotoli P., Brigida S., Calabrese A., Di Vittorio G., Lovero G., Caggiano G., Uricchio V.F, & Montagna M.T. (2016). Microbiological and hydrogeological assessment of groundwater in southern Italy. Environmental Monitoring and Assessment, 188(11), 638.
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3

Enterococci Identification from Melted Ice

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A 100 mL aliquot of melted ice was filtered, the membrane was placed on Slanetz and Bartley agar medium (Biolife Italiana Srl, Milan, Italy) and incubated at 36 ± 1 °C for 48 h. When dark pink-red colonies developed the membrane was transferred to a plate containing Bile Esculin Azide agar medium (Biolife Italiana Srl, Milan, Italy) and incubated at 44 °C for 2 h. Brown colonies with brown-black halos and positive catalysis were identified as Enterococci [15 ].
Caggiano G., Marcotrigiano V., Trerotoli P., Diella G., Rutigliano S., Apollonio F., Marzella A., Triggiano F., Gramegna M., Lagravinese D., Sorrenti G.T., Magarelli P., Moscato U, & Montagna M.T. (2020). Food Hygiene Surveillance in Italy: Is Food Ice a Public Health Risk?. International Journal of Environmental Research and Public Health, 17(7), 2408.
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