All the chemicals used in the present studies were of synthetic grade and were obtained from Merck specialties Ltd. and HiMedia laboratories Pvt. Ltd, (Mumbai, India). The prepared products were analysed by FT/IR (JASCO FT/IR 4100 Spectrophotometer) using KBr pellets. An LC-MS column C6 (150 mm × 4.6 mm) with a 5 μm particle size (Shimadzu-Mass spectrophotometer) was used. The 1H NMR spectra were recorded on a Bruker 1H NMR 400 MHz using tetramethylsilane as an internal standard, and the chemical shifts were reported in δ ppm. The UV (Jasco V-630 Spectrophotometer) and elemental analyses for C, H, N and S were performed on a Perkin Elmer model 2400 CHNS/O analyser. A Shimadzu XRD 7000 was used for the study of the structural environment of the synthesized ligands and their metal complexes. The Faraday balance technique was employed for the measurement of magnetic susceptibility of the metal complexes. The in vitro antimicrobial activities against different bacterial and fungal pathogens were determined by the agar well diffusion method, sourced from IMTECH, India, Chandigarh.
2400 chns o analyser
Manufactured by PerkinElmer
Sourced in United States
The 2400 CHNS/O Analyser is a laboratory instrument designed to determine the elemental composition of organic and inorganic samples. It can measure the concentrations of carbon, hydrogen, nitrogen, sulfur, and oxygen in a wide range of materials.
Automatically generated - may contain errors
Lab products found in correlation
Xrd 7000, by Shimadzu (2 mentions)
1h nmr 400 mhz, by Bruker (2 mentions)
V 630 spectrophotometer, by PerkinElmer (2 mentions)
Ft ir 4100 spectrophotometer, by Jasco (1 mentions)
Mass spectrophotometer, by Shimadzu (1 mentions)
Du730, by Beckman Coulter (1 mentions)
Avance 400 mhz, by Bruker (1 mentions)
Gc ms system, by Hewlett-Packard (1 mentions)
Arx 300 mhz, by Bruker (1 mentions)
Ft ir 4100, by Jasco (1 mentions)
4 protocols using 2400 chns o analyser
1
Spectroscopic Analysis of Synthetic Compounds
2
Leaf Nutrient and Pigment Analysis
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Leaves were analysed for total N concentration using a Perkin Elmer 2400 CHNS/O analyser (Perkin Elmer, Waltham, MA, USA). Leaf total P was measured using the colorimetric molybdenum–ascorbic acid method (Murphy and Riley 1962 (link)) following tissue combustion for 4 h at 550 °C and subsequent acid digestion. Photosynthetic pigments were extracted using 0.1 g of frozen leaf tissue in 20 mL of 80 % aqueous acetone. Chlorophyll a (Chl a) and chlorophyll b (Chl b) concentrations were determined in a spectrophotometer (Beckman Coulter DU-730, Beckman Coulter, Inc., Brea, CA, USA), using three wavelengths (663, 647 and 470 nm). Concentrations of pigments (mg g−1 DW) were obtained through calculation (Lichtenthaler 1987 (link)).
3
Iridium(I) Complex Synthesis Protocol
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All experiments were carried out under an inert atmosphere using standard Schlenk techniques. The solvents were dried by known procedures and distilled under argon prior to use or obtained oxygen- and water-free from a Solvent Purification System (Innovative Technologies). The starting complex was prepared according to a literature procedure [Ir(COD)(IPr)(acetone)][BF4].25f All other commercially available starting materials were purchased from Sigma-Aldrich, Merck and J. T. Baker and were used without further purification. H2 gas (>99.5%) was obtained from Infra.
1H, 13C{1H}, 19F, 1H–29Si HMBC, 1H–13C HMBC, 1H–13C HSQC and 1H–1H COSY NMR spectra were recorded either on a Bruker ARX 300 MHz or a Bruker Avance 400 MHz instrument. Chemical shifts (expressed in parts per million) are referenced to residual solvent peaks for 1H and 13C{1H}, and to an external reference of CFCl3 for 19F. Coupling constants, J, are given in Hz. Spectral assignments were achieved by combination of 1H–1H COSY, 13C APT and 1H–13C HSQC/HMBC experiments. C, H, and N analyses were carried out in a Perkin-Elmer 2400 CHNS/O analyser. GC-MS spectra were recorded on a Hewlett-Packard GC-MS system. Column chromatography was performed using silica gel (70–230 mesh).
1H, 13C{1H}, 19F, 1H–29Si HMBC, 1H–13C HMBC, 1H–13C HSQC and 1H–1H COSY NMR spectra were recorded either on a Bruker ARX 300 MHz or a Bruker Avance 400 MHz instrument. Chemical shifts (expressed in parts per million) are referenced to residual solvent peaks for 1H and 13C{1H}, and to an external reference of CFCl3 for 19F. Coupling constants, J, are given in Hz. Spectral assignments were achieved by combination of 1H–1H COSY, 13C APT and 1H–13C HSQC/HMBC experiments. C, H, and N analyses were carried out in a Perkin-Elmer 2400 CHNS/O analyser. GC-MS spectra were recorded on a Hewlett-Packard GC-MS system. Column chromatography was performed using silica gel (70–230 mesh).
4
Synthesis and Characterization of Novel Azo Compound
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All the chemicals (Merck specialties Ltd., Mumbai, India) used were of synthesis grade. Melting points were determined by the open capillary method (Elico), and were uncorrected. An FT/IR spectrophotometer was used to record the IR spectra of the synthesized molecules (JASCO FT/IR 4100, JASCO, JAPAN). A mass spectrophotometer with a C6 column (150 mm × 4.6 mm, 5 μm) was used to determine the molecular mass (Shimadzu). 1H NMR spectra were recorded using tetramethylsilane as an internal standard (Bruker 1H NMR, 400 MHz), and chemical shifts (δ) were reported in ppm. UV and elemental analyses were performed using a JASCO V-630 spectrophotometer and a Perkin Elmer 2400 CHNS/O analyser, respectively. XRD analysis was performed using a Cu Kα X-ray source; step = 0.02(2θ), run 2θ = 2–80°, scanning speed = 2°/min (Shimadzu XRD 7000). Structure elucidation was performed using the Origin data analysis software. A scanning electron microscope (EGOMA 15 ZEISS) was used to study the structural environment. Magnetic susceptibility was analysed by a Faraday balance.
The synthesis of 5-[(4-bromo-3-methylphenyl)diazenyl]-2-hydroxybenzaldehyde (4e ) was carried out as previously reported.6
The synthesis of 5-[(4-bromo-3-methylphenyl)diazenyl]-2-hydroxybenzaldehyde (
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