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Intensilight epifluorescence source

Manufactured by Nikon
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The Intensilight epifluorescence source is a compact, high-intensity fluorescence illumination system designed for microscopy applications. It provides a stable and uniform illumination for exciting fluorescent samples, enabling high-quality fluorescence imaging.

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Lab products found in correlation

Cfi apo 100, by Nikon (3 mentions) Eclipse ti, by Nikon (3 mentions) Perfect focus system, by Nikon (2 mentions) Optosplit 3, by Cairn Research (1 mentions) Elements software, by Nikon (1 mentions)

Close spelling variants of this product

Intensilight

6 protocols using intensilight epifluorescence source

1

Live Cell Imaging with Nikon Ti Microscope

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Live cells were imaged in standard cell media at 37 °C, and fixed cells
were imaged in 1% BSA in 1× PBS at room temperature. During imaging,
physiological temperature was maintained with a warming apparatus consisting of a sample
warmer and an objective warmer (Warner Instruments 641674D and 640375). The microscope was
a Nikon Eclipse Ti driven by the Elements software package. The microscope features an
Evolve electron multiplying charge coupled device (EMCCD; Photometrics), an Intensilight
epifluorescence source (Nikon), a CFI Apo 100× (numerical aperture (NA) 1.49)
objective (Nikon) and a TIRF launcher with two laser lines: 488 nm (10 mW) and 638 nm (20
mW). This microscope also includes the Nikon Perfect Focus System, an interferometry-based
focus lock that allowed the capture of multipoint and time-lapse images without loss of
focus. The microscope was equipped with the following Chroma filter cubes: TIRF 488,
TRITC, Epi 640 and reflection interference contrast microscopy (RICM).
Zhang Y., Ge C., Zhu C, & Salaita K. (2014). DNA-based digital tension probes reveal integrin forces during early cell adhesion. Nature communications, 5, 5167.
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2

Advanced Microscopic Imaging Techniques

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Epi fluorescence microscopy and TIRF microscopy experiments were performed on a Nikon Eclipse Ti inverted microscope driven by the NIS Elements software. The microscope features an Evolve electron-multiplying charge-coupled device (Photometrics), an Intensilight epifluorescence source (Nikon), a CFI Apo 100 X (numerical aperture 1.49) objective (Nikon) and a total internal reflection fluorescence launcher with three laser lines: 488 (10 mW), 561 (50 mW), and 638 nm (20 mW). This microscope also includes the Nikon Perfect Focus System, an interferometry-based focus lock which allows the capture of multi-point and time-lapse images without loss of focus. Cell-SSLB and cell-cell experiments were imaged on a Nikon confocal microscope. This microscope is equipped with a 60 X oil objective and a C2si scan head. Experiments were performed using three laser lines (488 nm, 561 nm and 640 nm) and the filters with the following bandpasses: 445/50+60LP, 525/50, and 600/50 nm. Z-stack imaging was performed using the ND Acquisition module in Nikon Elements.
Hu Y., Duan Y., Velusamy A., Narum S., Rogers J, & Salaita K. (2023). DNA Origami Tension Sensors (DOTS) to study T cell receptor mechanics at membrane junctions. bioRxiv.
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3

Single-molecule EGFR internalization imaging

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Images were acquired on a Nikon Eclipse Ti TIRF microscope with Elements software (Nikon). The microscope is equipped with an Evolve electron-multiplying CCD (charge-coupled device; Photometrics) used for cell imaging and a CoolSnap CCD camera (Photometrics) used for bead imaging, an Intensilight epifluorescence source (Nikon), a CFI Apo × 100 1.49 numerical aperture objective, and 488 nm (10 mW) and 638 nm (20 mW) laser lines. The microscope was equipped with a Quad Band (405/488/561/638m) laser TIRF filter cube with emission bands 500/25 and 730/55, and a cube for RICM (Chroma). EGFR internalization was imaged with an optical splitter, the Opto-Split III (Cairn Research), using 500/25 and 730/55 emission filters and a 600lp dichroic to split the fluorescence emission onto separate regions of the camera.
Stabley D.R., Oh T., Simon S.M., Mattheyses A.L, & Salaita K. (2015). Real-time fluorescence imaging with 20 nm axial resolution. Nature Communications, 6, 8307.
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4

Multimodal Microscopy Imaging Setup

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The microscope was a Nikon Eclipse Ti driven by the Elements software package. The microscope features an Evolve electron-multiplying charge-coupled device (Photometrics), an Intensilight epifluorescence source (Nikon), a CFI Apo 100× (numerical aperture 1.49) objective (Nikon) and a total internal reflection fluorescence launcher with three laser lines: 488 (10 mW), 561 (50 mW), and 638 nm (20 mW). This microscope also includes the Nikon Perfect Focus System, an interferometry-based focus lock that allowed the capture of multipoint and time-lapse images without loss of focus. In all the reported experiments, we used the following Chroma filter cubes: TRITC, Cy5, and RICM.
Ma V.P., Hu Y., Kellner A.V., Brockman J.M., Velusamy A., Blanchard A.T., Evavold B.D., Alon R, & Salaita K. (2022). The magnitude of LFA-1/ICAM-1 forces fine-tune TCR-triggered T cell activation. Science Advances, 8(8), eabg4485.
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5

Live Cell Imaging Setup and Configuration

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For live cell imaging at 37 °C, a Nikon Eclipse Ti microscope with Elements software was used, along with the warming apparatus consisting of an objective warmer and sample warmer. The microscope features a TIRF launcher with three laser lines: 488 nm (10 mW), 561 (50 mW), and 647 nm (20 mW), an Intensilight epifluorescence source (Nikon), an Evolve electron multiplying charge coupled device (EMCCD camera, Photometrics), and the Nikon Perfect Focus system that prevents losing focus during imaging. The microscope is equipped with TIRF 488, TIRF 640, FITC, TRITC, and RICM (reflection interference contrast microscopy) filter cubes that were purchased from Chroma (Bellows Falls, VT).
Galior K., Ma V.P., Liu Y., Su H., Baker N., Panettieri R J.r., Wongtrakool C, & Salaita K. (2018). Molecular Tension Probes to Investigate the Mechanopharmacology of Single Cells: A Step Towards Personalized Mechano-medicine. Advanced healthcare materials, 7(14), e1800069.
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6

Live Cell Imaging with Nikon Ti Microscope

Check if the same lab product or an alternative is used in the 5 most similar protocols
Live cells were imaged in standard cell media at 37 °C, and fixed cells
were imaged in 1% BSA in 1× PBS at room temperature. During imaging,
physiological temperature was maintained with a warming apparatus consisting of a sample
warmer and an objective warmer (Warner Instruments 641674D and 640375). The microscope was
a Nikon Eclipse Ti driven by the Elements software package. The microscope features an
Evolve electron multiplying charge coupled device (EMCCD; Photometrics), an Intensilight
epifluorescence source (Nikon), a CFI Apo 100× (numerical aperture (NA) 1.49)
objective (Nikon) and a TIRF launcher with two laser lines: 488 nm (10 mW) and 638 nm (20
mW). This microscope also includes the Nikon Perfect Focus System, an interferometry-based
focus lock that allowed the capture of multipoint and time-lapse images without loss of
focus. The microscope was equipped with the following Chroma filter cubes: TIRF 488,
TRITC, Epi 640 and reflection interference contrast microscopy (RICM).
Zhang Y., Ge C., Zhu C, & Salaita K. (2014). DNA-based digital tension probes reveal integrin forces during early cell adhesion. Nature communications, 5, 5167.
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