Beckman system gold instrument

Different bioactive compounds (inositol phosphates, α‐galactosides, and trypsin inhibitors,) were analyzed in the raw and treated Judión and Ayocote samples. Inositol phosphates were analyzed according to Pedrosa et al. (2015 (link)) using a HPLC (Beckman System Gold Instrument, Los Angeles, CA, USA) and a PRP‐1 column (150 × 4.1 mm i.d., 5 µm, Hamilton, Reno, NV, USA). Individual inositol phosphates (IP3–IP6) were quantified using an external standard (Sodium phytate; Sigma‐Aldrich, St. Louis, MO, USA). α‐Galactosides were analyzed according to Pedrosa et al. (2015 (link)) using a HPLC (Beckman System Gold Instrument, Los Angeles, CA, USA) equipped with a refractive index detector and a Spherisorb‐5‐NH2 column (250 × 4.6 mm i.d., Waters, Milford, MA, USA). The individual sugars were quantified by external standards (Sigma‐Aldrich, St. Louis, MO, USA). Trypsin inhibitors were obtained and determined as described by Pedrosa et al. (2015 (link)) and trypsin inhibitor units (TIU) were determined using α‐N‐benzoyl‐DL‐arginine‐p‐nitroanilide hydrochloride () as the trypsin substrate. One trypsin unit (TIU) was defined as that which gave a reduction in absorbance units at 410 nm of 0.01 relative to trypsin control reactions, using a 10 mL assay volume.