The paraffin embedded tissues were sliced (5 μm) and stained with haematoxy-lin-eosin. Frozen sections (5 μm) were stained with rat anti-CD8α (eBioscience, San Diego, CA) or rat IgG2a control antibody (eBioscience, San Diego, CA) and then developed with the Polink-1 HRP detection system for rat primary antibodies (ZSGB-BIO, Beijing). The slides were scanned with a Leica SCN 400 (Leica Camera, Allendale, NJ) and images were analysed by using SlidePath Gateway (Leica Microsystems Inc.).
Rat anti cd8α
Manufactured by Thermo Fisher Scientific
Sourced in Macao
The Rat anti-CD8α is a monoclonal antibody that specifically binds to the CD8α subunit of the CD8 protein, which is expressed on the surface of certain T cells. This antibody can be used to identify and isolate CD8+ T cells in various applications, such as flow cytometry and cell sorting.
Automatically generated - may contain errors
Lab products found in correlation
Donkey serum, by Merck Group (4 mentions)
Goat anti hfix, by Affinity Biologicals (4 mentions)
Alexa fluor 488 donkey anti rat, by Thermo Fisher Scientific (3 mentions)
Alexa fluor 568 donkey anti goat, by Thermo Fisher Scientific (3 mentions)
Rat igg2a control antibody, by Thermo Fisher Scientific (1 mentions)
E800 microscope, by Nikon (1 mentions)
Eclipse e800 microscope, by Nikon (1 mentions)
5 protocols using rat anti cd8α
1
Paraffin and Frozen Tissue Staining
2
Immunohistochemical Analysis of Muscle Tissue
3
Immunohistochemistry of Muscle Tissue
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Immunohistochemistry was performed using fluorescent antibodies on frozen and cryosectioned tissue, as previously described
[25 (link)]. Briefly, muscle tissue was harvested and frozen in liquid N2-cooled 2-methylbutane. Cryosections (10 μm) of tissue were fixed in acetone at room temperature, blocked with 5% donkey serum (Sigma, St. Louis, MO), and stained with rat anti-CD8α (eBioscience, San Diego, CA) and goat anti-hF.IX (Affinity Biologicals, Ontario, Canada). Secondary antibody donkey anti-rat Alexa Fluor 488 and donkey anti-goat Alexa Fluor 568 (Life Technologies, Eugene, OR) were used for detection. Fluorescence microscopy was performed with a Nikon E800 microscope (Nikon, Tokyo, Japan).
[25 (link)]. Briefly, muscle tissue was harvested and frozen in liquid N2-cooled 2-methylbutane. Cryosections (10 μm) of tissue were fixed in acetone at room temperature, blocked with 5% donkey serum (Sigma, St. Louis, MO), and stained with rat anti-CD8α (eBioscience, San Diego, CA) and goat anti-hF.IX (Affinity Biologicals, Ontario, Canada). Secondary antibody donkey anti-rat Alexa Fluor 488 and donkey anti-goat Alexa Fluor 568 (Life Technologies, Eugene, OR) were used for detection. Fluorescence microscopy was performed with a Nikon E800 microscope (Nikon, Tokyo, Japan).
4
Immunofluorescence Analysis of Mouse Muscle Tissue
5
Immunohistochemical Analysis of Muscle Cryosections
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Immunohistochemistry was performed on cryo-sections of quadricep muscle, as previously described (30 (link)). Briefly, cryosections (10 μm) of muscle were fixed in pre-cooled acetone at −20°C, blocked with 5% donkey serum (Sigma, St. Louis, MO) and stained with rat anti-CD8α (eBioscience) and goat anti-hFIX (Affinity Biologicals, Ontario, CA) antibodies at room temperature. Secondary antibodies, donkey anti-rat conjugated to Alexa Fluor 488 and donkey anti-goat conjugated to Alexa Fluor 568 (Life Technologies, Carlsbad, CA, USA) were used for detection. Sections were mounted using Prolong Diamond antifade with DAPI mounting media (Invitrogen, Carlsbad, CA). Mounted sections were stored protected from light at 4°C until visualization and image acquisition.
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