Viabilities of colon cancer cells in different groups were measured using the Cell Counting Kit-8 (CCK-8; AbMole, USA). According to the protocols, cells (5×103/well) were inoculated in 96-well plates. After being incubated for 12, 24, and 48 h, cells were stained with 10 μl CCK-8 staining reagent (final concentration at 10 nmol/L) for 1 h. The optical density (OD) values were measured using a 1500 microplate reader (Thermo, USA) at 450 nm.
1500 microplate reader
Manufactured by Thermo Fisher Scientific
Sourced in United States
The 1500 microplate reader is a laboratory instrument designed for absorbance-based assays. It is capable of reading microplates with up to 96 wells and can measure absorbance at multiple wavelengths. The device is intended for use in various applications that require quantitative analysis of samples in a microplate format.
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Lab products found in correlation
4 protocols using 1500 microplate reader
1
Colorimetric Assay for Colon Cancer
2
Cell Viability Assessment via CCK-8
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Cell Counting Kit-8 (CCK-8; Beyotime Institute of Biotechnology, Haimen, China) was applied to determine cell viabilities. To be more specific, cells (5×103 /well) were inoculated in 96-well plates. After being incubated for 24 h (h), cells were stained with 20 µl staining reagent for 1 h. The optical density (OD) values at 450 nm were measured by 1500 microplate reader (Thermo Fisher Scientific, Inc., Waltham, MA, USA).
3
Quantifying Colon Cytokines in Vitro
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The colons were cut into 1 cm pieces and homogenate with the cold PBS. After centrifugation at 1200 rpm (5424R, Eppendorf) for 20 min at 4 °C, the supernatant was carefully collected. The levels of IL-1β and IL-18 in the supernatant and the in vitro culture were quantified measured by using the kits according to the manufacturer's instruments. The optical densities were read on a Microplate Reader 1500 (Thermo Fischer) at 450 and 570 nm. The levels of IL-1β and IL-18 in vitro culture or in colons are presented as pg/mL or pg/mg, respectively.
4
Evaluating Cell Viability with MTT Assay
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The viability of cells was assessed by using the MTT reagent. Briefly, 1 × 106 cells/mL BMDMs and PMA-differentiated THP-1 cells were seeded into 96-well plates, and treated with lonicerin (1, 3, 10, and 30 μmol/L) for 20 h. Afterwards, viable cell were stained with 20 μL of MTT solution (5 mg/mL) for another 4 h. Finally, the supernatant was removed, and the absorbance of formazan crystal dissolved in 150 μL dimethyl sulfoxidedmwas (DMSO) measured at 570 nm by using Microplate Reader 1500 (Thermo Fischer, Waltham, MA, USA).
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