All primary antibodies used in the present study are listed in Star Methods Table S1 . Secondary horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit antibodies were from BioRad. All secondary antibodies for immunofluorescence were from goat host, conjugated to Alexa-dyes (Life Technologies). Exclusively isotype-specific secondary antibodies were used against monoclonal mouse antibodies. Alexa555-tagged Phalloidin was from Life Technologies.
Synthetic peptides were purchased from ChinaPeptides (Shanghai, China). Calmidazolium and W7 were bought from Tocris, and TFP from MP Biomedicals. All reagents used for cloning were obtained from New England Biolabs (NEB). If not specifically stated, all other reagents were of standard quality and from the usual vendors.
Synthetic peptides were purchased from ChinaPeptides (Shanghai, China). Calmidazolium and W7 were bought from Tocris, and TFP from MP Biomedicals. All reagents used for cloning were obtained from New England Biolabs (NEB). If not specifically stated, all other reagents were of standard quality and from the usual vendors.