High glucose (25 mM) Dulbecco's modified eagle's medium (DMEM; D5796), cell culture grade water, N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (dbcAMP), dexamethasone (Dex) and 3,3′,5-Triiodo-l -thyronine sodium salt (T3) were all purchased from Sigma-Aldrich (Poole, UK). Human Recombinant Des(1–3) IGF-I was purchased from SourceBioscience and other cell culture reagents were purchased from Fisher Scientific (Loughborough, UK). Compounds were prepared as follows: dbcAMP was dissolved in cell culture grade water at 100 mM, Des(1–3) IGF-I in 0.1 M acetic acid at 100 μg/ml, T3 in 0.1 M sodium hydroxide at 100 nM and Dex in absolute ethanol at 10 mM. Compounds were filter-sterilised through a 0.2 μm filter and aliquots stored at −20 °C.
3 3 5 triiodo l thyronine sodium salt t3
Manufactured by Merck Group
Sourced in United Kingdom, France
3,3′,5-Triiodo-l-thyronine sodium salt (T3) is a synthetic form of the thyroid hormone triiodothyronine. It is commonly used in research and laboratory settings as a reference standard or analytical control.
Automatically generated - may contain errors
Lab products found in correlation
D glucose, by Merck Group (2 mentions)
N6 2 o dibutyryladenosine 3 5 cyclic monophosphate sodium salt dbcamp, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem d5796, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Putrescine, by Merck Group (1 mentions)
Progesterone, by Merck Group (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Sodium selenite, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
Collagenase type 5, by Merck Group (1 mentions)
Ham s f10 medium, by Thermo Fisher Scientific (1 mentions)
Human canine porcine insulin quantikine elisa kit, by R&D Systems (1 mentions)
Calcium chloride dihydrate, by Merck Group (1 mentions)
Isobutylmethylxanthine, by Merck Group (1 mentions)
N 2 hydroxyethylpiperazine n 2 ethanesulfonic acid hepes, by Merck Group (1 mentions)
Dna mini kit, by Qiagen (1 mentions)
Snakeskin dialysis tubing 10 000 mwco, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
7 protocols using 3 3 5 triiodo l thyronine sodium salt t3
1
Differentiation of Adipocytes with Hormones
2
Myelin Differentiation Induction in Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified and expanded SCs were treated with 4 mM N6,2′-O-Dibutyryladenosine3′,5′-cyclic monophosphate sodium salt (dB-cAMP) for 48 h to induce up regulation of myelin differentiation markers after which they were seeded and then cultured overnight in starvation medium.**** DMEM/F12 (Gibco®) with SATO [(100 μg/mL bovine serum albumin (BSA; Sigma®), 100 μg/mL transferrin powder (Sigma®), 16 μg/mL Putrescine; 60 ng/mL progesterone (Sigma®) and 40 ng/mL sodium selenite (Sigma®) in Neurobasal medium (Gibco®)], 1X B27 supplement; 0.101 mg/mL of 3.3′,5-Triiodo-L -thyronine sodium salt (T3, Sigma®), 10 mM Insulin, 1% L -glutamine and 1% A/A) supplemented with 0.5% FBS.
3
Synthesis and Characterization of Polymeric Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NHS-PEG-b-PLA copolymers and Mal-PEG-b-PLA copolymers with a molecular weight of 6000 Da were purchased from Nanosoft Polymers. DiI was purchased from Biotium. Ham’s F10 medium and bovine serum albumin (BSA) were obtained from Gibco and GenDEPOT, respectively. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotic-antimycotics were purchased from Biowest. The DNA Mini Kit and RNeasy Mini Kit were purchased from QIAGEN. TOPscript™ complementary DNA (cDNA) Synthesis Kit and qPCR SyGreen Mix were purchased from Enzynomics and PCR BIOSYSTEMS, respectively. SnakeSkin™ Dialysis Tubing (10,000 MWCO) was purchased from Thermo and pluriStrainer® 500 µm was purchased from pluriSelect. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay kit was obtained from iNtRON Biotechnology. Hank’s balanced salt solution (HBSS), dimethyl sulfoxide (DMSO), nicotinamide, D-glucose, L-glutamine, calcium chloride dihydrate, isobutylmethylxanthine, ciprofloxacin, N-(2-Hydroxyethyl) piperazine-N′-(2-ethanesulfonic acid) (HEPES), sodium bicarbonate, collagenase type V, acridine orange (AO), propidium iodide (PI), and 3,3',5-triiodo-L-thyronine sodium salt (T3) were purchased from Sigma Aldrich. Human/Canine/Porcine Insulin Quantikine ELISA Kit purchased from R&D Systems. All other chemicals and reagents were of analytical grade.
4
Polymer-based Drug Delivery System Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Poly(l -lactide) (PLLA) (RESOMER® L 206 S, inherent viscosity = 0.8–1.2 dL/g), Poly(d ,l -lactide-co-glycolide) 50:50 (PLGA 50:50) (RESOMER® RG 504 H, inherent viscosity = 0.45–0.60 dL/g), PLGA 75:25 (RESOMER® RG 756 S, inherent viscosity = 0.71–1.0 dL/g) and PLGA 85:15 (RESOMER® RG 858 S, inherent viscosity = 1.3–1.7 dL/g) were purchased from Evonik Industries. N,N-Dimethylformamide (DMF), dichloromethane (DCM), and methanol (MeOH) were purchased from Sigma Aldrich. 3,3’,5-Triiodo-l -thyronine sodium salt (T3) and Ibuprofen (Ibu) were purchased from Sigma Aldrich.
For HPLC analysis, acetonitrile was of HPLC grade and was purchased from Sigma Aldrich. For LC-MS/MS analysis, methanol, acetonitrile, and formic acid were of LC-MS grade and were purchased from Sigma Aldrich (St. Louis, MO, USA). Ultrapure water was produced in-house with a Human Power I system (Seoul, Korea). Oasis® HLB 200 mg SPE cartridges were purchased from Waters (Milford, MA, USA).
For HPLC analysis, acetonitrile was of HPLC grade and was purchased from Sigma Aldrich. For LC-MS/MS analysis, methanol, acetonitrile, and formic acid were of LC-MS grade and were purchased from Sigma Aldrich (St. Louis, MO, USA). Ultrapure water was produced in-house with a Human Power I system (Seoul, Korea). Oasis® HLB 200 mg SPE cartridges were purchased from Waters (Milford, MA, USA).
5
Oli-neu Cells Exposure to BPA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oli-neu cells were maintained in DMEM, high glucose GlutaMAXTM Supplement, pyruvate (Gibco reference 31966047, Thermo Fisher Scientific, Courtaboeuf, France) supplemented with 1% N2 (Gibco reference 17502001, Thermo Fisher Scientific, Courtaboeuf, France), 1% heat inactivated horse serum (Gibco reference 26050070, Thermo Fisher Scientific, Courtaboeuf, France), 1% penicillin-streptomycin (10,000 U/mL) (Gibco reference 15140122, Thermo Fisher Scientific, Courtaboeuf, France), 10 µg/mL insulin from bovine pancreas (Sigma I5500, Saint Quentin Fallavier, France), 0.4 µg/mL L-Thyroxine sodium salt pentahydrate (T4) (Sigma T0397, Saint Quentin Fallavier, France,), and 0.34 µg/mL 3,3′,5-Triiodo-L-thyronine sodium salt (T3) (Sigma T6397 Saint Quentin Fallavier, France).
Flasks and wells were coated with poly-L-lysine (Sigma P4707, Saint Quentin Fallavier, France) diluted at 20 µg/mL. Cells were exposed to BPA (Sigma 239658, Saint Quentin Fallavier, France) during 72 h of proliferation and an additional 72 h of differentiation induced by 1 μM PD174265 in DMSO (Sigma 513040-M, Saint Quentin Fallavier, France). The medium was changed after 48 h of differentiation. Oli-neu cells were exposed to 0.01, 0.1, 1, 10, 20, 50, and 100 µM BPA in DMSO. The final concentration of DMSO was 0.2%.
Flasks and wells were coated with poly-L-lysine (Sigma P4707, Saint Quentin Fallavier, France) diluted at 20 µg/mL. Cells were exposed to BPA (Sigma 239658, Saint Quentin Fallavier, France) during 72 h of proliferation and an additional 72 h of differentiation induced by 1 μM PD174265 in DMSO (Sigma 513040-M, Saint Quentin Fallavier, France). The medium was changed after 48 h of differentiation. Oli-neu cells were exposed to 0.01, 0.1, 1, 10, 20, 50, and 100 µM BPA in DMSO. The final concentration of DMSO was 0.2%.
6
Receptor Activation Pathway Modulation
7
Chemicals and Reagents for Cell Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
D(+)-galactose and D(+)-raffinose were purchased from ACROS Organics (Geel, Belgium). Yeast nitrogen base without amino acids and Bacto peptone were purchased from BD Biosciences (Franklin lakes, NJ). D(+)-glucose, Bacto yeast extract, dimethylsulfoxyde (vehicle control), 3,3′5-triiodo-L-thyronine sodium salt (T3), Nutlin-3, Nutlin-3a, FK-506 monohydrate, and the different amino acids complements were purchased from Sigma (St. Louis, MO).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!