7000c gc ms triple quad

GC-TQ-MS/MS was performed on an Agilent 7890B GC system equipped with a 7000 C GC/MS Triple Quad. For the DB-5MS (15 m × 0.25 mm × 0.1 μm) capillary column, splitless injection was performed at 250 °C. The GC oven temperature was adjusted according to the following program: an initial temperature of 50 °C was maintained for 1 min, followed by an increase to 200 °C at a rate of 40 °C·min⁻¹, an increase to 240 °C at a rate of 20 °C·min⁻¹, an increase to 246 °C at a rate of 1.5 °C·min⁻¹, and an increase to 300 °C at a rate of 40 °C·min⁻¹, with the final temperature being maintained for 3 min. For the DB-5MS (30 m × 0.25 mm × 0.1 μm) column, the oven temperature program was as follows: 50 °C isothermal for 1 min, increased to 240 °C at 50 °C·min⁻¹, increased to 255 °C at 1.5 °C·min⁻¹, increased to 300 °C at 50 °C·min⁻¹ and maintained at the final temperature for 1 min. Helium was used as the carrier gas at a flow rate of 1 mL·min⁻¹. The inlet temperature was set to 300 °C, the ion trap temperature was 250 °C, the electron energy was 70 eV, and the spectra were recorded over a range of 10-400 m/z. Qualitative Analysis software (B.07.00) was used for data analysis.

Transfected HeLa and MCF7 cells were harvested and washed two twice with ice‐cold PBS. Lipid was extracted using 1 000 μl of extracting solution made using ice‐cold methanol, acetonitrile, choloroform and water at ratio of 2:2:2:1 (v/v). The lower phase containing lipid was then isolated and air dried using rotary evaporator. Dried lipids were resuspended in N,O‐Bis(trimethylsilyl)trifluoroacetamide (BSTFA) containing 1% trimethylsilyl choloride (TMCS). Lipids were mixed well and heated to 60°C for up to 30 min until all liquid content is evaporated. Followed by cooling at room temperature and then resuspended in chloroform. Derivatised lipids were injected into gas chromatography‐tandem mass spectroscopy (GC‐MS; 7000C GC/MS Triple Quad, Agilent Technologies).

Gas chromatography (Agilent 7000C GC/MS Triple Quad, Santa Clara, CA, USA) equipped with an HP-5MS column (length = 30 m; diameter = 0.25 mm; film thickness = 0.25 m) mass spectrometer programmed at temperature 30–280/300 °C with a hold time of 5 min and rate of 10 °C/min was used to investigate bioactive compounds in methanol extract of dried Arctostaphylos uva-ursi. The chromatography conditions were as follows: column flow rate of 1 mL/min, injection mode split, and carrier gas Helium 99.999%. GC-MS spectra with mass library search (National Institute of Standards and Technology, Bureau Drive Gaithersburg, MD, USA, based AMDIS software) and relative retention indices were used to identify the components [42 (link)].