2-Amino-5,6-dihydro-6-methyl-4H-1,3-thiazine hydrochloride (AMT) was purchased from Tocris Cookson Ltd. (Bristol, UK). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dexamethasone, IL-1β, and LPS (Escherichia coli 0127:B8) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). SP600125 (JNK inhibitor) was obtained from Tocris Bioscience (Bristol, UK). RPMI and other cell culture reagents including FBS were products of Thermo Scientific Hyclone (Logan, UT, USA). All primary antibodies including mitogen-activated protein kinase (MAPK) antibodies and transcription factor antibodies were obtained from Cell Signaling Technologies (Danvers, MA, USA). Lamin antibody was purchased from Bioworld Technology (Louis Park, MN, USA). The protein assay kit was purchased from Bio-Rad Lab. (Hercules, CA, USA).
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dexamethasone
Manufactured by Merck Group
Sourced in United States
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a chemical compound used in cell-based assays to assess cell viability and proliferation. Dexamethasone is a synthetic glucocorticoid medication used as an anti-inflammatory and immunosuppressant agent.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Il 1β, by Merck Group (2 mentions)
Protein assay kit, by Bio-Rad (2 mentions)
Sp600125, by Bio-Techne (1 mentions)
Lps escherichia coli 0127 b8, by Merck Group (1 mentions)
β actin antibody, by Fortis Life Sciences (1 mentions)
Interleukin il 1β, by Merck Group (1 mentions)
Lamin b1 antibody, by Bioworld Technology (1 mentions)
Ab182563, by Abcam (1 mentions)
Hrp linked anti rabbit igg, by Cell Signaling Technology (1 mentions)
Hrp linked anti mouse igg, by Cell Signaling Technology (1 mentions)
Recombinant human il 1β, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Welgene (1 mentions)
Hdac4, by Cell Signaling Technology (1 mentions)
Lamin a c, by Cell Signaling Technology (1 mentions)
β catenin, by Cell Signaling Technology (1 mentions)
Runx2, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
β actin, by Merck Group (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
4 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dexamethasone
1
Molecular Mechanisms of Anti-Inflammatory Effects
2
Anti-inflammatory Compound K Effects
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2-Amino-5,6-dihydro-6-methyl-4H-1,3-thiazine hydrochloride (AMT) was purchased from Tocris Cookson Ltd. (Avonmouth, Bristol, UK). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dexamethasone, interleukin (IL)-1β, and lipopolysaccharide (LPS, Escherichia coli 0127:B8) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Compound K-enriched fraction (5%) was obtained from Fleton Natural Products Co. (Chengdu, China). The protein assay kit was purchased from Bio-Rad Lab (Hercules, CA, USA). All antibodies relating to MAPK and nuclear transcription factor-κB (NF-κB) signaling were purchased from Cell Signaling Technologies (Dancers, MA, USA). β-actin antibody was obtained from Bethyl Laboratories, Inc. (Montgomery, TX, USA). Lamin B1 antibody was purchased from Bioworld technology (Minneapolis, MN, USA).
3
Chondrogenic Differentiation of Mesenchymal Stem Cells
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Dulbecco’s modified Eagle medium (DMEM) and Penicillin-streptomycin from Welgene (Namcheon-myeon, Gyeongsangbuk-do, Korea). Fetal bovine serum (FBS) from Gibco (Carlsbad, CA, USA). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), dexamethasone, and lithium chloride from Sigma-Aldrich (St. Louis, MO, USA). Recombinant Human IL-1β from PeproTech (Cranbury, NJ, USA). H89 (#9844) and Okadiac acid (#5934) from Cell Signaling Technology (Bervely, MA, USA). Parathyroid Hormone (7-34), Human Recombinant (228-11343-2) from RayBiotech (Peachtree Corners, GA, USA). Antibodies against VEGFA (ab46154, 1:1000) and Collagen X (ab182563, 1:1000) from Abcam (Cambridge, UK). RUNX2 (#12566, 1:1000), HDAC4 (#5392, 1:1000), SOX9 (#82630, 1:1000), β-catenin (#8480, 1:1000), Lamin A/C (#4777, 1:2000), HRP linked anti-mouse IgG (#7076, 1:5000), and HRP linked anti-rabbit IgG (#7074, 1:5000) from Cell Signaling Technology (Bervely, MA, USA). β-actin (A5411, 1:5000) from Sigma Aldrich (St. Louis, MO, USA). Antibody Collagen II (sc-52658, 1:1000) and PTH/PTHrP-R (sc-12722, 1:1000) from Santa Cruz Biotechnology (Dallas, TX, USA). HSP90 (13171-1-1AP, 1:2500) from Proteintech (Rosemont, IL, USA).
4
Inflammatory Response Modulation Assessment
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2-Amino-5,6-dihydro-6-methyl-4H-1,3-thiazine hydrochloride (AMT) was purchased from Tocris Cookson Ltd (Bristol, UK). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dexamethasone, IL-1β and lipopolysaccharide (LPS) (Escherichia coli 0127:B8) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI 1640 and other cell culture reagents including fetal bovine serum (FBS) were products of Gibco BRL (Grand Island, NY, USA). IL-1β mouse ELISA kit and IL-6 Human ELISA kit were purchased from BD bioscience (San Jose, CA, USA).
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