Rabbit anti mouse phospho gsk3β ser389

For western blot analysis, rabbit anti-human γH2AX (Ser139) (1:1000, #9718S, lot 10), rabbit anti-human GSK3β (1:15,000, #9315, lot 12), rabbit anti-human p38 MAPK (1:1000, #9212, lot 11) and rabbit anti-human phospho-p38 MAPK (Thr180/Tyr182) (1:1000, #9215, lot 7) were obtained from Cell Signaling (Danvers, MA). Rabbit anti-mouse phospho-GSK3β (Ser389) (1:3000, #07-2275, lot 2272702) was obtained from Millipore (Billerica, MA, USA). Blots were normalized using mouse anti-chicken α-tubulin (1:50,000, #T6199, Sigma, St. Louis, MO). For western blots, goat anti-rabbit IgG (H + L) (#111-035-144) and goat anti-mouse IgG (H + L) (#115-035-146) horseradish peroxidase-conjugated secondary antibodies were utilized (1:3000, Jackson ImmunoResearch, West Grove, PA, USA). For immunohistochemistry, Rabbit anti-mouse phospho-GSK3β (Ser389) (1:400, Millipore) and mouse antihuman γH2AX (Ser139) (1:500, Millipore #05-636, lot 2476967, the same lot as used in (Thornton et al., 2016 )) antibodies were utilized. For single γH2AX labeling, Cy3-conjugated donkey anti-mouse IgG (H + L) (#715-165-150) was used, while for dual γH2AX and phospho-GSK3β co-localization, DyLight 488-conjugated donkey anti-mouse IgG (H + L) (#715-485-151) and Cy3-conjugated donkey anti-rabbit IgG (H + L) (#711-165-152) secondary antibodies were utilized (1:500, Jackson ImmunoResearch).