The levels of urea produced by CHME5 and Imhu cells were detected by the QuantiChrom Urea Assay kit (BIOassay System, Hayward, CA, USA), used according to the manufacturer’s instructions. Briefly, an aliquot of cell culture media (50 μL) was mixed with 200 μL Urea Reagent (Bioassay system) and the absorbance measured at 430 nm in a spectrophotometric microplate reader (PerkinElmer). A standard curve was generated during each assay in the range of concentrations 0–100 μg/ml using urea as standard. In this range, the detection of urea resulted linear, and the minimum detectable concentration was 3.12 μg/mL. Protein content in each sample was determined by Bradford method (Bio-Rad Laboratories) using BSA as standard.
Quantichrom urea assay kit
Manufactured by BioAssay Systems
Sourced in United States, Canada
The QuantiChrom Urea Assay Kit is a colorimetric assay that measures the urea concentration in biological samples. It provides a simple and accurate method for the quantification of urea.
Automatically generated - may contain errors
Lab products found in correlation
Quantichrom creatinine assay kit, by BioAssay Systems (21 mentions)
Mouse albumin elisa kit, by Fortis Life Sciences (7 mentions)
Urea reagent, by BioAssay Systems (4 mentions)
Albuwell m kit, by Exocell (4 mentions)
Elisa quantitation kit, by Fortis Life Sciences (3 mentions)
Creatinine enzymatic reagent set, by Horiba (3 mentions)
Spectrophotometric microplate reader, by PerkinElmer (3 mentions)
Human albumin elisa kit, by Fortis Life Sciences (3 mentions)
Bradford method, by Bio-Rad (2 mentions)
Tamoxifen, by Merck Group (2 mentions)
Spectramax m5, by Molecular Devices (2 mentions)
Kge005, by R&D Systems (2 mentions)
Coomassie blue stain solution, by Bio-Rad (2 mentions)
Albuwell m, by Exocell (2 mentions)
Creatinine assay kit, by R&D Systems (2 mentions)
Pierce bca assay, by Thermo Fisher Scientific (2 mentions)
Mouse albumin elisa quantification kit, by Fortis Life Sciences (2 mentions)
Nh4cl, by Merck Group (2 mentions)
In cell analyzer 2000, by GE Healthcare (2 mentions)
P450 glo cyp3a4 assay kit, by Promega (2 mentions)
201 protocols using quantichrom urea assay kit
1
Quantifying Urea Levels in Cell Cultures
2
Generating ADPKD Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
Crtc2−/− mice and Pkd1flox/flox; Cre/Esr1+ mice were described previously.[71 , 72 ] The authors generated Pkd1flox/flox; Crtc2−/− mice by crossing Pkd1flox/flox; Cre/Esr1+ mice with Crtc2−/− mice. The activity of Cre recombinase was induced by intraperitoneal injection of tamoxifen (Sigma, T5648) at P10 (10 mg per kg [body weight]) to generate ADPKD mouse model. The kidney tissues were collected on P29. Total area and cyst area measurement were evaluated using the National Institutes of Health ImageJ software. The cyst index was calculated as the ratio of cyst area to total area. Blood urea nitrogen (BUN) was measured in plasma using the QuantiChrom Urea Assay Kit (DIUR, BioAssay Systems). All mice care and experimental protocols were approved by the Ethical Committee of Tianjin Medical University (permit number: SYXK 2009‐00001).
+ Expand
Crtc2−/− mice and Pkd1flox/flox; Cre/Esr1+ mice were described previously.[
3
Measuring Albumin and MPO in Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Albumin concentration was measured with the bromocresol green method [14 ], and MPO was measured in whole TA as previously reported [15 (link)]. TA dilution was calculated by analysing plasma and TA urea levels using a commercial kit (QuantiChrom urea assay kit; Bioassay System, Hayward, CA, USA). The ratio between plasma urea and TA urea reflected TA dilution [16 (link)].
4
Macrophage Arginase Activity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
50µl of lysed macrophages were transferred in a new 96-well round bottom plate, 50µl of activation solution were added (10mM MnCl2, 50mM TrisCl, pH=7.5) and incubated for 10 minutes at 55°C. 25µl of each well were transferred in a new 96-well round bottom plate, 25µl of substrate solution (0.5M L-arginine, pH = 9.7) were added and incubated for one hour at 37°C. 50µl of each well were transferred in a new 96-well round bottom plate, 200µl of reagent from the QuantiChrom urea assay kit (BioAssay systems, reference DIUR-100) were added and incubated 20 minutes at room temperature in the dark. Absorbance was read at 520nm and results were calculated according to manufacturer’s instructions.
5
Serum Biomarker Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum hepcidin concentrations were determined using enzyme-linked immunosorbent assay as previously detailed.11 (link) Serum Erfe concentrations were determined using the Mouse ERFE enzyme–linked immunosorbent assay Kit (Intrinsic LifeSciences), serum EPO using the Quantikine Mouse EPO Kit (R&D Systems), serum urea nitrogen using the QuantiChrom Urea Assay Kit (BioAssay Systems), serum creatinine using the Mouse Creatinine Kit (Crystal Chem), and serum lactate dehydrogenase using the Lactate Dehydrogenase Activity Kit (Sigma-Aldrich).
6
Evaluation of Hepatocyte Function and Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was determined immediately after thawing using the trypan blue exclusion test. Mitochondrial dehydrogenase activity, cell attachment, and hepatocyte specific function were evaluated after maintenance in culture for 24 h. For mitochondrial dehydrogenase activity and cell attachment assay, cells were cultured in collagen-coated 96-well plates (5.0 × 104 cells/well). The mitochondrial dehydrogenase activity of hepatocytes was assessed using an MTT assay.29 (link) Hepatocyte attachment was assessed using the sulforhodamine B (SRB) assay.30 (link) Albumin and urea synthesis were assessed for cells cultured in collagen-coated 24-well plates (3.0 × 105 cells/well). Albumin production was quantified in supernatant using human or rat albumin enzyme-linked immunosorbent assay (ELISA) quantitation kits (Bethyl Laboratories, Montgomery, TX, USA). Urea synthesis was assessed after challenging hepatocytes with ammonium chloride (5 mM) for 6 h at 37 °C. Supernatant was collected and analyzed using the QuantiChrom™ Urea Assay Kit (BioAssay Systems, Hayward, CA, USA).
7
Nitric Oxide and Urea Quantification
8
Quantitative Urea Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BUN was measured following the manufacturer’s protocol (QuantiChrom Urea Assay Kit, BioAssay Systems, 501078333).
9
Bioanalysis of Serum Biochemical Markers
10
Blood Serum BUN Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse blood samples were collected and placed at room temperature for 40 min. The whole blood samples were centrifuged at 4000 rpm for 10min at 4°C. Serum was collected and stored at 4°C. Serum BUN levels were detected using QuantiChrom™ Urea Assay Kit (DIUR-100, BioAssay Systems) according to the manufacturer’s protocol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!