MTT assays were introduced into 96-well plates containing 100 μl complete medium (3000 cells/well) and incubated for three days at 37°C in 5% CO2. After three days, the medium was replaced by 20 μl MTT solution (Solarbio, Beijing, China, 5 mg/ml) and incubated for 4 hours at 37°C. Then, MTT solution was removed and 150 μl DMSO was added (Solarbio, Beijing, China) to every well for 10 minutes at room temperature to solubilize the formazan crystals. The optical density (OD) was detected by using a spectrophotometer at 570 nm.
Mtt solution
Manufactured by Solarbio
Sourced in China, United States
MTT solution is a laboratory reagent used for cell viability and proliferation assays. It is a yellow tetrazolium salt that is reduced by metabolically active cells to form purple formazan crystals. The intensity of the purple color is proportional to the number of viable cells, making MTT solution a useful tool for quantifying cell growth and cytotoxicity.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (24 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (11 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (10 mentions)
Microplate reader, by Bio-Rad (7 mentions)
Microplate spectrophotometer, by Agilent Technologies (4 mentions)
Microplate reader, by Agilent Technologies (4 mentions)
Mtt solution, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Beyotime (2 mentions)
Thermo multiskan plate reader, by Thermo Fisher Scientific (2 mentions)
Spectramax m3, by Molecular Devices (2 mentions)
Automatic enzyme labeled reading meter, by Thermo Fisher Scientific (2 mentions)
96 well plate, by Corning (2 mentions)
Microplate reader, by Molecular Devices (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Solarbio (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
Adriamycin, by Meilun (1 mentions)
Mtt kit, by Solarbio (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Im0280, by Solarbio (1 mentions)
93 protocols using mtt solution
1
MTT Assay for Cell Viability
2
Evaluating Cell Viability via MTT Assay
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Viability of cells was evaluated by the MTT assay. In brief, the transfected cells were sorted in 96-well plates at 3000 cells per well. Forty-eight hours later, each well was loaded with 20 μL MTT solution (5 mg/mL, Solarbio Science & Technology Co., Ltd., Beijing, China). Following another 3 h of incubation at 37 °C, the supernatant was discarded and the precipitated formazan was dissolved in 150 μL dimethyl sulphoxide (DMSO) solution. Then, the optical density (OD) value was determined at 490 nm using a microplate spectrophotometer.
3
MTT Assay for Cell Viability
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Cells were injected into 96‐well plates, and MTT solution (M1025, Solarbio, Beijing, China) was added into each well after incubation for 24 hours, 48 hours, 72 hours and 96 hours. After incubation for another four hours, dimethyl sulfoxide (DMSO; Solarbio) was added into each well. The absorbance was monitored by Microplate Reader (Bio‐Rad, Hercules, CA, USA) at 490 nm.
4
MTT Assay for Cell Viability
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Cell viability was examined via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H -tetrazolium bromide (MTT) analysis. 1 × 104 SNU-387 and Huh7 cells were added into 96-well plates and incubated for 48 h. Next, the MTT solution (Solarbio) was placed into each well with a final concentration of 0.5 mg/mL, and cells were cultured for 4 h. Then, the medium was removed and 100 μL of dimethyl sulfoxide (DMSO; Beyotime, Shanghai, China) was added. The absorbance at 570 nm was determined with a microplate reader (Molecular Devices, Sunnyvale, CA, USA). Cell viability was normalized to the control group.
5
MTT Assay for Cell Viability
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Cell viability was determined by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Solarbio, Guangzhou, People’s Republic of China) colorimetric assay. In short, cells were seeded into 96-well plates and treated for 72 h with different concentrations of rapamycin (0, 10, 25 and 100 nM). After treatment, cells were washed with PBS and incubated in MTT solution (Solarbio) for 4 h. Dimethyl sulfoxide was then added to each well, and absorbance was measured at 490 nm with a microplate reader (BioRad, CA, United States).
6
MTT Assay for Cell Viability
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Cell viability was measured using an MTT assay. Cells were seeded in a 96-well plate at a density of 1x103 cells/well in 100 µl of culture medium and incubated at 37˚C with 5% CO2 for 24 h. After treatment with different concentrations of TMZ for 48 h, 5 mg/ml MTT solution (Beijing Solarbio Science & Technology Co., Ltd.) was added to each group at the appropriate time and incubated for a further 4 h at 37˚C in the dark, after which 200 µl DMSO was added to dissolve the purple crystals. Cell viability was detected at 450 nm using a microplate reader (Infinite M200 Pro; Tecan Group). IC50 was calculated using GraphPad Prism version 5.01 (GraphPad Software, Inc.) by plotting a nonlinear regression curve fit using log (inhibitor) vs. normalized response (variable slope). Experiments were performed in triplicate.
7
Cell Proliferation Assay with MTT
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Exponentially growing cells were prepared as a single cell suspension. Cells were seeded in 96-well plates (3000 cells/well) and assessed at time points of 0, 24, 48, 72 and 96 h. Time ‘0’ indicated 6 h post plating. Following addition of 20 µl of MTT solution (5 µg/ml, Cat#M8180, Solarbio, Beijing, China) to each well, the cells were incubated at 37 °C for 4 h. After removing the culture medium, 150 µl of dimethyl sulfoxide (DMSO, Solarbio, Beijing, China) was added to each well, and the plate was incubated with shaking for 5 min. Optical density (OD) was measured at 490 nm. The experiment was performed in triplicate.
8
MTT Assay for Cell Viability
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The cells were seeded into 96-well plates (5 × 103 cells/well) after transfection. After 24 h, the cells were exposed to a single-dose of irradiation. After different durations of incubation, 20 μl of 0.5 mg/ml MTT solution (Solarbio, Beijing, China) was added into each well and the cells were incubated for 4 h at 37 °C. The supernatant solution was then removed and 200 µl of DMSO was added into each well. Absorbance of the supernatant solution was measured using a microplate reader (Bio-Rad, Hercules, CA, USA) at a wavelength of 490 nm after 15 min of vibration.
9
MTT Assay for Cell Viability
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MCF 10A (3 × 103 cells per well) or HCC1937 (3.2 × 103 cells per well) and MDA-MB-231 (2.9 × 103 cells per well) cells were seeded in 96-well plates and incubated for 48 h. Twenty microliters of the MTT solution (Solarbio, China) were added to each well and incubated for 2 h at 37°C in a 5% CO2 incubator. The cell culture medium was removed and then 200 μl of DMSO were added to each well and crystals were dissolved. Absorbance was measured at 570 nm using the Thermo Multiskan plate-reader (Thermo Fisher Scientific).
10
Cell Proliferation Assays of SW1088 and SW1733
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Cell proliferation assays were performed using SW1088 and SW1733 cells in a 96-well plate, which are gift from Dr. Jingwen Jiang’s lab. The cells were seeded at a density of 3000 cells/well in 100 µl of 10% FBS DMEM. For the Cell Counting Kit-8 (CCK-8) assays, the original medium in each group was replaced with 10 µl of CCK-8 solution diluted in 100 µl of complete culture medium, following the protocol provided by Biosharp. After an additional 1-h incubation in the dark at 37 °C, the viable cells were detected by measuring the absorbance at a wavelength of 450 nm. For the MTT assays, 10 µl of MTT solution (Solarbio) was added to each well after 48 h of cell culture. After a 2-h incubation, the medium was discarded, and the optical density was measured at 490 nm using a Microplate Reader.
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