Pen strep neomycin

EMT6 murine breast carcinoma, LLC murine Lewis lung carcinoma, and RENCA murine renal cell carcinoma cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and were used within 6 months of resuscitation. Cells were routinely tested to be mycoplasma-free. Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, SigmaAldrich, Rehovot, Israel), and supplemented with 5% fetal calf serum (FCS), 1% L-glutamine, 1% sodium pyruvate, and 1% Pen-Strep-Neomycin in solution, (Biological Industries, Israel). All cells were cultured in a humidified chamber in 5% CO₂ at 37°C.

4T1, EMT6 (murine breast carcinoma cell lines), RENCA (murine renal carcinoma), and LLC (murine Lewis lung carcinoma) were purchased from the American Type Culture Collection (Manassas, VA, USA) and were used within 6 months of thawing. Cells were routinely tested to be mycoplasma-free. All the cell lines were maintained under 37°C and 5% CO₂ conditions in Dulbecco's modified Eagle's medium (DMEM, Sigma-Aldrich, Rehovot, Israel, Cat# D5796) supplemented with 10% fetal calf serum (FCS, Biological Industries, Israel, Cat# 10270-106), 1% L-glutamine (Cat# 03-020-1B), 1% sodium pyruvate (Cat# S8636), and 1% Pen-Strep-Neomycin (Cat# 03-034-1B) in solution (Biological Industries, Israel).