Acetonitrile

CH₂Cl₂, methanol, acetonitrile, isopropyl alcohol, and formic acid are HPLC grade from Sinopharm Chemical Reagent Co. (Shanghai, China). Ethanol, trinitrophenol, xylene, n-butanol, and formaldehyde are AR grade from FUYU Chemical Co. (Tianjin, China).

High-fidelity DNA polymerase was purchased from TSINGKE Biological Technology (Beijing, China), a One-step PCR Cloning Kit was purchased from Novoprotein Scientific (Shanghai, China), restriction endonucleases were purchased from Thermo Fisher Scientific (Massachusetts, United States), and the kits used for plasmid and RNA extraction were obtained from Generay Biotech (Shanghai, China). Other chemicals were purchased as follows: epothilone standard samples, Meilun Biotechnology (Dalian, China); methanol (MACLIN, China), acetonitrile (Sinopharm Chemical, China) and Amberlite™ XAD-16 resin (Mosu, Shanghai, China). The primers used in this study were synthesized by TSINGKE Biological Technology and Generay Biotech, and the genes were sequenced by TSINGKE Biological Technology and GENEWIZ (Suzhou, China).

Ferrous sulfate, bismuth nitrate, sodium borohydride, anhydrous ethanol, citric acid (CA), sodium citrate (NaCA), glacial acetic acid, methanol, acetonitrile, ferric nitrate, sulfamethazine (SM2), sulfamethoxazole (SMX), and sulfadiazine (SD) were supplied by Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China). The 5-dimethyl-1-pyrroline N-oxide (DMPO) was purchased from Aladdin Industrial Company (Shanghai, China). methanol and acetonitrile were HPLC grade, while other chemical reagents were analytical grade. All aqueous solutions were prepared using ultrapure water.

Bovine serum albumin (BSA), tetramethylbenzidine, and horseradish peroxidase were obtained from Merck (Merck, Darmstadt, Germany). Goat anti-mouse IgG was purchased from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). Nicarbazin was obtained from Biovet JSC (Peshtera, Bulgaria). Chloramphenicol, trimethoprim, and sulphanilamide were purchased from J&K Scientific Ltd. (Beijing, China). Coating antigen and monoclonal antibody 14D2 were prepared and provided by the Xiya Zhang research team at Henan Agricultural University. Trisodium citrate, aurichlorohydric acid, K₂CO₃, methanol, acetonitrile, and other chemical reagents were provided by Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China). The chicken sample was purchased from Darunfa Market (Hefei, China) and was verified to be negative by liquid chromatography–mass spectrometry.
Nitrocellulose membrane (Millipore 135) was obtained from Millipore AG (Millipore AG, Zug, Switzerland). A 96-well microplate was purchased from Yunpeng Technology Development Co., Ltd. (Xiamen, China). Blood filtration membrane, polyvinyl chloride plates, and absorbent paper were purchased from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). A Milli-Q Ultrapure water meter was obtained from Millipore (Massachusetts, USA). An HQ-6-Ⅱ vortex mixer was bought from Beijing North Tongzheng Biotechnology Development Co., Ltd. (Beijing, China).

The sheets of manchurian ash (MA) with a thickness of 4.3 mm were purchased from Shaoxing Fengsen Wood Co., Ltd. (Shaoxing, China), which were used as the sole precursor material for the preparation of all-wood biocomposites. MA was cut into sheets with size of 55 × 40 × 4.3 mm and dried in a vacuum oven at 100 °C for 24 h prior to use. 1-Butyl-3-methylimidazolium chloride (BMIMCl) with a purity of ≥95%, purchased from J&K Scientific Ltd. (Beijing, China), was used as the solvent of cellulose in MA. The melting point of BMIMCl was 73 °C. It was also dried in a vacuum oven at 100 °C for 24 h before being utilized in order to remove residual moisture. Acetonitrile with a grade of analytical reagent (purity ≥99.0%) was provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), and it was used to remove BMIMCl in the composites after hot-pressing.