Sv 40 renilla luc

Manufactured by Promega

Sourced in United States

The SV-40-Renilla-Luc is a plasmid that contains the SV-40 promoter, the Renilla luciferase gene, and the firefly luciferase gene. It is designed for the expression and measurement of both Renilla and firefly luciferase activities in mammalian cells.

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Lab products found in correlation

Dual luciferase assay kit, by Promega (5 mentions) Dual luciferase assay system, by Promega (2 mentions) Superfect transfection reagent, by Qiagen (2 mentions) C fos luc, by Promega (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Luciferase reporter assay system, by Promega (1 mentions) Passive lysis buffer (plb), by Promega (1 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Ap 1 luc, by Promega (1 mentions) Pnf κb luc, by Promega (1 mentions)

Close spelling variants of this product

SV-40 Renilla-Luc-plasmid (2 citations)

8 protocols using sv 40 renilla luc

Dual-Luciferase Assay for NF-κB Activity

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Cells cultured in six-well plates were transfected with an empty vector, lin28b-P1-Luc containing an NF-κB binding site (GGGGCTTTC) in the first intron, or a mutant lin28b-P1(GGGGCTTTC→TCATACGAT)-Luc (2 (link), 5 (link)) and, as an internal control, SV-40-Renilla-Luc (Promega) in the presence of Superfect transfection reagent (Qiagen). After 48 h, the transfected cells were left untreated or treated with the indicated agents, lysed with passive lysis buffer and then the lysates were analyzed using the dual luciferase assay system (Promega).

Alam M., Ahmad R., Rajabi H, & Kufe D. (2014). MUC1-C INDUCES THE LIN28B→LET-7→HMGA2 AXIS TO REGULATE SELF-RENEWAL IN NSCLC. Molecular cancer research : MCR, 13(3), 449-460.

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Transfection-Mediated Luciferase Assay

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293 cells were transfected with c-fos-Luc and SV-40-Renilla-Luc (Promega, Madison, WI) with CDK2 or ELK4 in the presence of transfection agent. At 30 h after transfection, cells were disrupted in passive lysis buffer and lysates analyzed for firefly and Renilla luciferase activities using the dual luciferase assay kit (Promega).

Peng C., Zeng W., Su J., Kuang Y., He Y., Zhao S., Zhang J., Ma W., Bode A.M., Dong Z, & Chen X. (2015). Cyclin dependent kinase 2 (CDK2) is a key mediator for EGF-induced cell transformation mediated through the ELK4/c-Fos signaling pathway. Oncogene, 35(9), 1170-1179.

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Dual Luciferase Assay for Transfected Cells

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Cells were cultured in six-well plates followed by transfection with an empty vector, pCRB3-Luc and, as an internal control, SV-40-Renilla-Luc (Promega) in the presence of Superfect transfection reagent (Qiagen). After 48 h, the transfected cells were lysed with passive lysis buffer and the lysates were analyzed using the Dual Luciferase Assay system (Promega).

Alam M., Bouillez A., Tagde A., Ahmad R., Rajabi H., Maeda T., Hiraki M., Suzuki Y, & Kufe D. (2016). MUC1-C Represses the Crumbs Complex Polarity Factor CRB3 and Downregulates the Hippo Pathway. Molecular cancer research : MCR, 14(12), 1266-1276.

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STAT3 Transcriptional Activity Assay

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Approximately 1 × 10⁶ Hela cells were transfected with p-STAT3-Luc (Panomics) and SV40-Renilla-Luc (Promega) using Lipofectamine 2000 (Invitrogen,USA). Twenty-four hours later, the cells were pretreated with YL064 for 1 h, and IL-6 (R&D Systems, Minneapolis, MN, USA, 20 ng/ml) was added and incubated for additional 4 h. Then, the cells were collected and lysed in passive lysis buffer (Promega) for 15 min, the transcriptional activity of STAT3 was determined using a luciferase reporter assay system (Promega), according to the manufacturer’s instructions.

Wang Y., Wu L., Cai H., Lei H., Ma C.M., Yang L., Xu H., Zhu Q., Yao Z, & Wu Y. (2018). YL064 directly inhibits STAT3 activity to induce apoptosis of multiple myeloma cells. Cell Death Discovery, 4, 44.

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EGF-Induced Transcriptional Activation

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Cells were transfected with AP-1-Luc and SV-40-Renilla-Luc (Promega, Madison, WI). After 20 h transfection, cells were continued to culture with serum-free medium for 16 h and then treated with EGF (100 ng/ml) for indicated time points. Cell lysates were analyzed by firefly and Renilla luciferase activities with a dual-luciferase assay kit (Promega) following protocol.

Zhang X., Wu L., Xiao T., Tang L., Jia X., Guo Y., Zhang J., Li J., He Y., Su J., Zhao S., Tao J., Zhou J., Chen X, & Peng C. (2018). TRAF6 regulates EGF-induced cell transformation and cSCC malignant phenotype through CD147/EGFR. Oncogenesis, 7(2), 17.

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NF-κB Transcriptional Activity Assay

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Cells were transfected with pNF-κB-Luc and SV-40-Renilla-Luc (Promega, Madison, WI). At 20 h post transfection, the cells were serum-starved for 16 h, pretreated with EGCG for 1 h, and then treated with IL-1beta (20 ng/ml). The cells were then collected in passive lysis buffer. The cell lysates were analyzed for their firefly and Renilla luciferase activities using a dual luciferase assay kit (Promega).

Zhang J., Lei Z., Huang Z., Zhang X., Zhou Y., Luo Z., Zeng W., Su J., Peng C, & Chen X. (2016). Epigallocatechin-3-gallate(EGCG) suppresses melanoma cell growth and metastasis by targeting TRAF6 activity. Oncotarget, 7(48), 79557-79571.

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Notch Signaling Activation in MSCs

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80% confluence MSCs were transfected with Notch responsive RBPJ-Luc and SV40-Renilla-Luc (Promega) in the presence of Lipofectamine 2000 (Invitrogen) followed by OA treatment. At 24 hours after transfection and treatment, lysates were analyzed with a Dual Luciferase Assay Kit (Promega).

Shu B., Zhao Y., Wang Y., Wang G., Shang X., Britt M., Olmedo M., Chelly M., Morandi M.M., Barton S, & Dong Y. (2017). Oleanolic Acid Enhances Mesenchymal Stromal Cell Osteogenic Potential by Inhibition of Notch Signaling. Scientific Reports, 7, 7002.

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Transfection-Mediated Luciferase Assay

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