Paraffin-embedded liver tissue samples were obtained from the same 28 patients described above, and then sliced and deparaffinized according to standard procedures. The slides were incubated with anti-PCK1 antibody at 4°C overnight. After washing with PBS, the sections were incubated with secondary anti-rabbit IgG (ZSGB-BIO, Beijing, China) and stained with 3,3'-diaminobenzidine (ZSGB-BIO). For immunohistochemical assessment, the staining images of 3 pairs of HCC and adjacent non-tumor tissues were quantified using integrated optical density (IOD) by Image-Pro Plus 6.0 software.
Secondary anti rabbit igg
Manufactured by ZSGB-BIO
Sourced in China
The Secondary anti-rabbit IgG is a laboratory reagent used to detect and quantify the presence of rabbit immunoglobulin G (IgG) in biological samples. It functions by binding to rabbit IgG molecules, enabling their identification and measurement in various experimental and diagnostic procedures.
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Lab products found in correlation
5 protocols using secondary anti rabbit igg
1
Quantitative Immunohistochemistry of PCK1 in HCC
2
Histological Analysis of Liver Samples
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Liver samples were fixed in fresh 4% paraformaldehyde and subjected to routine histological procedures for embedding in paraffin. Then, the samples were cut in 4.5 μm-thick sections that were processed for hematoxylin and eosin (HE) or IHC staining with antibodies targeting PCK1 (1:500), pAMPK (1:200), or p27Kip1 (1:500). For IHC assays, the sections were incubated with secondary anti-rabbit IgG (ZSGB-BIO, Beijing, China) and stained with 3,3′-diaminobenzidine (ZSGB-BIO). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images were acquired using Pannoramic Viewer 1.15.2 (3DHistech, Budapest, Hungary).
3
Immunohistochemical Analysis of Hepatitis B Virus Core Antigen
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After fixation in 4% paraformaldehyde for 24 h, liver tissue samples were embedded in paraffin according to standard procedures. The resultant sections were incubated with anti-HBcAg (Cat. no. B0586; Dako, Glostrup, Denmark) separately. Subsequently, the slides were incubated with secondary anti-rabbit IgG (Cat. no. ZB-2301; ZSGB-BIO, Beijing, China) and visualized using 3, 3′-diaminobenzidine (ZSGB-BIO). After rinsing, the samples were dehydrated, treated with xylene for transparency, and scanned with an Olympus BX61 microscope.
4
Immunohistochemical Analysis of Liver Samples
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Liver samples were fixed in fresh 4% paraformaldehyde and subjected to routine histological procedures for embedding in paraffin. Then, the samples were cut in to 4.5-μm sections, which were processed for hematoxylin and eosin (HE) staining or IHC staining with antibodies targeting PCK1(1:500), and β-catenin (1:500). For IHC assay, the sections were incubated with secondary anti-rabbit IgG (ZSGB-BIO, Beijing, China) and stained with 3,3′-diaminobenzidine (ZSGB-BIO). Stained slides were scanned with a Pannoramic Scan 250 Flash or MIDI system and images were acquired using Pannoramic Viewer 1.15.2 (3DHistech, Budapest, Hungary).
5
Immunohistochemical Analysis of β-Catenin
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Liver tissues were fixed with 4% paraformaldehyde, embedded in paraffin, and sectioned according to standard procedures. Tissue slices were incubated with a β‐catenin antibody at 4°C overnight. Subsequently, the slides were incubated with a secondary anti‐rabbit IgG (ZSGB‐BIO, Beijing, China) and visualized using 3,3′‐diaminobenzidine (ZSGB‐BIO).
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