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Kyse70

Manufactured by Cobioer Biosciences
Sourced in China

The KYSE70 is a laboratory equipment designed for cell culture applications. It is a multi-functional incubator that provides precise temperature, humidity, and CO2 control for cell growth and maintenance. The KYSE70 is suitable for a variety of cell lines and supports consistent and reliable cell culture conditions.

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2 protocols using kyse70

1

Authentication and Cultivation of Human ESCC Cell Lines

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Human ESCC cell lines KYSE150, KYSE70, KYSE180, and KYSE520 were obtained from CoBioer Biosciences Co, Ltd, (Nanjing, China) and authenticated by a third-party short tandem repeat analysis (Genetic Testing Biotechnology, Suzhou, China). All cell lines were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin and incubated at 37 °C in a humidified atmosphere of 5% CO2.
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2

Esophageal Squamous Cell Carcinoma Cell Lines

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The normal esophageal epithelial cell line (NE3), originally developed at Hong Kong University, was obtained from Professor Li Fu (Department of Pharmacology, Shenzhen University). Other human ESCC cell lines, including Eca-109 (RRID: CVCL_6898), KYSE-30 (RRID: CVCL_1351), KYSE-70 (RRID: CVCL_1356), KYSE-180 (RRID: CVCL_1349), KYSE-450 (RRID: CVCL_1353), and TE1 (RRID: CVCL_1759) were purchased from the Cobioer Biosciences Co., Nanjing, China. NE3, Eca-109, KYSE30, KYSE70, KYSE180, KYSE450, and TE1 cells were maintained in 10% FCS-RPMI-1640 medium (Invitrogen, Shanghai, China). NE3 cells were cultured in EpiCM 4101 medium (ScienCell, USA). The cells were cultured in humidified atmosphere of 5% CO2 at 37°C. All cell lines were authenticated by matching the short-tandem repeat (STR) DNA profiles of the cell to the corresponding standard STR in the database of ATCC (the American Type Culture Collection) and DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen). All cells were routinely tested and found negative for mycoplasma.
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