Bright field pictures of C. elegans gonads and cuticle blisters were taken using a digital camera attached to a Leica microscope. Pictures of unshedded cuticles were taken using a digital camera attached to an Olympus SZX16 dissecting microscope. Intestinal autofluorescence of excess iodide-treated animals were observed under an Olympus SZX16 dissecting microscope using either a GFP filter (excitation wavelength 460–495 nm) or an RFP filter (excitation wavelength 530–550 nm).
Szx16 dissecting microscope
Manufactured by Olympus
Sourced in Japan
The SZX16 is a dissecting microscope designed for detailed examination and observation of specimens. It features a zoom ratio of 16:1, providing a wide range of magnification from 0.75x to 11.25x. The microscope is equipped with a high-performance optical system that delivers clear, high-contrast images. The SZX16 is suitable for a variety of applications, including biological sample analysis, electronics assembly, and precision-based tasks.
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Lab products found in correlation
Vhx 5000 digital microscope, by Keyence (4 mentions)
Fv1000 confocal microscope, by Olympus (4 mentions)
Bx53 compound microscope, by Olympus (2 mentions)
Bx51 fluorescence microscope, by Olympus (2 mentions)
Crystal violet, by Merck Group (2 mentions)
Bx61 compound microscope, by Olympus (1 mentions)
Bx53 stereomicroscope, by Olympus (1 mentions)
Dulbecco modified eagle medium (dmem), by Boster Bio (1 mentions)
Matrigel, by BD (1 mentions)
Tricaine, by Merck Group (1 mentions)
Dp2 bsw software, by Olympus (1 mentions)
Acridine orange base, by Merck Group (1 mentions)
Bx51wi compound microscope, by Olympus (1 mentions)
Mz16 microscope, by Leica (1 mentions)
Axioskop, by Zeiss (1 mentions)
Szx16, by Olympus (1 mentions)
Vhx 5000, by Keyence (1 mentions)
Fv1000 microscope, by Olympus (1 mentions)
Dextran 568, by Thermo Fisher Scientific (1 mentions)
Igf 1, by Merck Group (1 mentions)
26 protocols using szx16 dissecting microscope
1
Microscopic Imaging of C. elegans
2
Lichen Metabolite Identification Protocol
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The specimens were collected from southern China and deposited in the Fungarium, College of Life Sciences, Liaocheng University, China (LCUF ). Morphological and anatomical characters of thalli and apothecia were examined and photographed under an Olympus SZX16 dissecting microscope and an Olympus BX53 compound microscope. The lichen secondary metabolites were detected and identified by thin-layer chromatography using solvent C (Orange et al. 2010 ; Jia and Wei 2016 ).
3
Arabidopsis Silique Sectioning and Embryo Dissection
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Siliques of Arabidopsis plants expressing TCOp::TCO-GUS were GUS-stained for 24 h as previously detailed [58 (link)], fixed overnight in FAA (3.7% formaldehyde, 50% ethanol, 5% glacial acetic acid), embedded in paraffin, and sectioned to a thickness of 20 µm. Sections were then deparaffinized, rehydrated through a reverse ethanol series (100% to 30%), incubated in water, mounted and imaged. For embryo dissection, seeds were fixed in 4% paraformaldehyde under vacuum for 2 h, rinsed three times in phosphate buffered saline, and incubated in clearing solution (6M urea, 30% glycerol, 0.1% Triton X-100) [63 (link)] for 3 weeks at 4 °C. Embryos were liberated from the seed coat by applying gentle pressure. An Olympus SZX16 dissecting microscope was used to capture images of live plant tissues, while an Olympus BX61 compound microscope was used to capture images of fixed/sectioned tissues and GFP fluorescence (Olympus, Center Valley, PA, USA).
4
Chicken Embryo Angiogenesis Assay
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The embryos of unhatched eggs from specific-pathogen-free white Leghorn chicken were placed laterally in an incubator (37 °C, relative humidity: 55–60 %). On day 4 of the incubation period, 2.5 mL of albumin was drawn from the eggs using a 20-G needle, and a fake air chamber was constructed on the embryo. Subsequently, the needle hole and fake air chamber were sealed using 3 M breathable tape. On day 7, CBT-143-S-F6F7 was dissolved with dimethyl sulfoxide (DMSO) and diluted with PBS. For each group, including a vehicle group, the final DMSO concentration was 1 %. CBT-143-S-F6F7 was loaded on 6-mm-diameter Circular Advantec filter paper (Toyo Roshi Kaisha, Ltd., Tokyo, Japan), and the paper was placed on the CAM. On day 9, the CAM was photographed using the SZX16 dissecting microscope (Olympus, Japan). By using the filter paper as the center, four concentric circles were marked on the photograph (diameters of 7, 8, 9, and 10 mm; total circumference of the four circles was 106.8 mm, and total circumference represented the region near the filter paper) [25 (link)]. The amount of vessels crossing the concentric circles (the vascular density index or VDI) was used to evaluate the state of angiogenesis. The VDI for the same photograph was determined by three people, and the mean VDI was adopted.
5
Morphological Study of Insect Specimens
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Morphological terminology used in this work follows Dietrich (2005) (link). Habitus photos were taken using a KEYENCE VHX-5000 digital microscope. Body length was measured from the apex of the vertex to the tip of the forewings. Abdomens were removed from specimens and cleared in cold 10% KOH solution overnight. The cleared material was rinsed with water and stored in glycerine. An Olympus SZX16 dissecting microscope was used for specimen study and Olympus BX53 stereoscopic microscopes were used for drawing of the dissected male genitalia and wings. All specimens examined are deposited in the collection of the School of Karst Science, Guizhou Normal University, China (GZNU
6
Methodology for Insect Specimen Preparation
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The specimen was collected by the sweeping-net method. Morphological terminology used follows Dietrich (2005) (link) and Dworakowska (1993) . An Olympus SZX16 dissecting microscope was used for observing and an Olympus BX53 stereomicroscope for drawing. A KEYENCE VHX-5000 digital microscope was used for taking habitus photos. Body measurements are measured from the apex of the vertex to the tip of the forewing. Male specimens were selected under a stereoscope, the entire abdomen of the specimens was removed and soaked in 10% sodium hydroxide (NaOH) solution or 10% potassium hydroxide (KOH) solution for 15-20 hours. After that, the abdomen was rinsed with clean water, drained of the excess water with qualitative filter paper and transferred to a clean glass dripping with glycerine. All specimens examined were deposited in the collection of the School of Karst Science, Guizhou Normal University/State Key Laboratory Cultivation Base for Guizhou Karst Mountain Ecology Environment of China, Guiyang, China.
7
Lymphatic Metastasis Model of Cervical Cancer
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Female athymic nude (nu/nu) 4-week-old mice (n=40) were purchased from Beijing HFK Bio-technology Co, Ltd. (Beijing, China). The studies were approved by the Committee on the Ethics of Animal Experiments of Tongji Medical College (Wuhan, China). Mice were maintained in the accredited animal facility of Tongji Medical College at 20–26°C in a 12-h light/dark cycle. A lymphatic metastasis model of cervical cancer was used as previously described (16 (link)). Briefly, 5×106 tumor cells in 50 µl serum-free DMEM (Boster Biological Technology, Co., Ltd., Wuhan, China)/Matrigel (BD Biosciences) at a 9:1 ratio were injected subcutaneously into the claw pads of the mice. Tumor size (mm3) was measured and calculated by the following formula: Volume = (width)2 × length / 2. When primary tumors reached ~150 mm3 in size, metastases were tracked by optical imaging of luciferase activity originating from tumor cells using the IVIS Spectrum system (Caliper Life Sciences; PerkinElmer, Inc., Waltham, MA, USA). Subsequently, when primary tumors reached ~250 mm3 in size, the mice were euthanized, and their popliteal and inguinal lymph nodes were excised. Metastases of tumor cells in the lymph nodes were confirmed by detection of tumor-expressed RFP under a SZX16 dissecting microscope (Olympus Corporation). The incidence of metastasis-positive mice in each group was calculated.
8
Aortic Hematoma Inspection Protocol
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After ketamine (100 mg/kg i.p.) and xylazine (10 mg/kg i.p.), a midline incision was performed, the abdomen and chest were opened widely, the circulation was flushed with saline, the viscera were excised, and the retroperitoneum entered. The suprarenal abdominal aorta was inspected for hematomas using a SZX16 dissecting microscope with a camera attachment (Olympus).
9
Zebrafish Emodin Angiogenesis Assay
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Transgenic Tg (Fli1a: EGFP) fish were raised at 28.5 °C under a 10/14 h dark/light cycle. The night before emodin treatment, male and female zebrafish were kept in the tank containing a fish mating cage (including an inner mesh and divider). Embryos were collected after natural spawning then rinsed with system water. The embryos or larvae were kept at 28.5 °C and incubated in E3 embryo medium (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, and 0.33 mM MgSO4, pH 7.2). At 12 h post fertilization, the medium was supplemented with 0.003% 1-phenyl-2-thiourea (PTU) at 28.5 °C to prevent pigment formation. After 72 h post fertilization, larvae were anesthetized with 0.168 mg/mL tricaine (Sigma-Aldrich), and photographed with a DP72 digital camera mounted on an SZX16 dissecting microscope (Olympus Corporation). All zebrafish experiments were approved by the Institutional Animal Care Committee of Nankai University and conformed to the National Institutes of Health Guidelines. The effects of emodin on ISV development were analyzed by χ2-test.
10
Quantitative Analysis of Embryo Phenotypes
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Images of the phenotypes of embryos or larvae and images of whole-mount in situ hybridisation were captured with a DP72 digital camera mounted on an SZX16 dissecting microscope (Olympus Corporation, Tokyo, Japan). DP2-BSW software (Olympus) was used to calculate the body lengths, eye sizes and areas of gh1/rag1-positive signals. Images of HE staining were photographed with a DP71 digital camera mounted on a BX51 fluorescence microscope (Olympus). Images of immunofluorescence were captured with an FV 1000 confocal microscope (Olympus). ImageJ software (1.49×; NIH, http://rsb.info.nih.gov/ij/ ) was used to convert the fluorescence images of Zpr1 or Zpr3 immunostaining to 8-bit greyscale prior to thresholding and calculate the area of positive areas in each image. All images were compiled in Adobe Photoshop CS6 Portable (Adobe Systems Incorporated, San Jose, CA, USA) and resized. All images involved in the experiment were similarly manipulated.
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