An X-ray generator (X-RAD 320 ix, Precision X-ray Inc., North Branford, CT, United States) was utilized to deliver radiation at a dose rate of 1.0 Gy/min.
X rad 320 ix
Manufactured by Precision X-Ray
Sourced in United States
The X-RAD 320 ix is a versatile x-ray irradiator designed for research and preclinical applications. It features a high-performance x-ray source capable of delivering a range of x-ray energies and dose rates. The system is equipped with advanced imaging capabilities and can be configured with various irradiation chambers to support a variety of experimental setups.
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Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Raw264.7 cells, by Thermo Fisher Scientific (1 mentions)
Cisplatin cddp, by Teva Pharmaceuticals (1 mentions)
Erastin, by Selleck Chemicals (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Hepg2, by National Collection of Authenticated Cell Cultures (1 mentions)
Mhcc 97h, by National Collection of Authenticated Cell Cultures (1 mentions)
Clinac 23ex, by Agilent Technologies (1 mentions)
18 protocols using x rad 320 ix
1
X-Ray Radiation Delivery Protocol
2
Hypoxia and Radiation Effects on Tumor Cells
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Mouse Lewis lung carcinoma cells were obtained from American Type Culture Collection. Mouse RAW264.7 macrophages were obtained from Cell Bank of Chinese Academy of Sciences (Shanghai, China). Lewis cells and RAW264.7 cells were cultured in DMEM medium, supplemented with and 10% fetal bovine serum (Gibco, USA). Cells were incubated at 37°C in a humidified incubator containing 5% CO2. For hypoxia exposure, cells were placed in a hypoxia chamber that maintained a low oxygen tension (1% O2, 5% CO2, and 94% N2). Cell irradiation was performed using X-RAD320ix (Precision X-Ray, North Branford, CT, USA) with a dose of 4 Gy at room temperature.
3
X-Ray Radiation Delivery Protocol
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An X-ray generator (X-RAD 320 ix, Precision X-ray Inc., North Branford, CT, United States) was utilized to deliver radiation at a dose rate of 1.0 Gy/min.
4
Optimized Treatment Conditions for Bortezomib and Cisplatin
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Bortezomib (BZM; Cell Signaling Technology, Danvers, MA) was diluted in dimethyl sulfoxide (DMSO, stock: 1 mM) according to the manufacturer's instructions and stored at −20°C upon use. Further dilution steps were carried out directly before application, and an equal dilution of DMSO was used as solvent control. Cisplatin (CDDP; TEVA, Ulm, Germany) was supplied as a stock solution (1 mg/ml) (Center for Cytostatics Preparation, University Hospital Gießen and Marburg, Germany) and further diluted in pure water (stock: 1 mM) directly before application. X-ray irradiation (IR) was carried out using an X-RAD 320 iX (Precision X-Ray Inc., Denver, CO) X-ray tube; anode voltage: 320 kV, current: 10 mA, dose rate: 1.2 Gy/min, focus object distance: 60 cm, filter: 0.5 mm Cu and 0.5 mm Al. Further treatment conditions are indicated in the results section.
5
PDAC Cell Irradiation Protocol
6
Dose-dependent Larval Irradiation Survival
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Actively crawling third instar larvae were irradiated with the dosage of 0, 10, 20, 30, and 40 Gy, respectively, at a dose rate of 1.3 Gy/min in a X-Ray Biological Irradiator (X-RAD 320iX, Precision X-ray Inc). At least 100 larvae were treated for each genotype at each dosage, and experiments were repeated three times. Survival percentage was calculated as the number of viable adults divided by the number of irradiated third instar larvae. Significant differences between the values under designated experimental conditions were subjected to two-tailed Student’s t-tests. For all tests, a P value less than 0.05 was considered statistically significant.
7
Ionizing Radiation Exposure in Larval Flies
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Actively crawling third instar larvae of w 1118 and atm stg flies were collected and irradiated with 40 Gy, at a dose rate of 340 cGy/min, with X-RAD 320 iX at 320 kV (Precision X-Ray, Inc., North Branford, CT). The irradiated L3 larvae were recovered for 1 h before further experiments.
8
Ionizing Radiation Sensitivity in Mre11 Mutants
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Actively crawling third instar larvae of w1118, mre11R559A1-1T10, mre11R563A3-1D5, mre11R565A2-7M5, mre11R569A6-34H1, mre114RA2-3P3 were collected, and irradiated at a rate of 1.3 Gy/min with dosage of 0, 10, 20, 30, 40 Gy in a X-Ray Biological Irradiator (X-RAD 320iX, Precision X-ray Inc), respectively. Let the irradiated flies grow to adult on fly medium, the number of viable flies was counted and survival percentage was calculated as viable flies divided by larvae irradiated. At least 100 larvae were treated at each dosage for each genotype, and experiments were repeated three times.
9
Radiation Response of A549 and H1299 Cells
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Flasks (75 cm2) with 60% confluent cells were laid with a 1 cm thick compensation above and 50 cm source surface distance (SSD) below, and the field size was 30×30 cm2. A549 and H1299 cells were irradiated with X-RAD320ix (Precision X-Ray, North Branford, CT, USA) Following the final radiation, the cells were maintained according to the above culture method in a humidified incubator with 5% CO2 at 37° C.
10
Xenograft Mouse Model of Pancreatic Cancer
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Six-week-old male BALB/c nude mice were obtained from Beijing Vitallihua. This study protocol was approved by the Institutional Animal Care and Usage Committee of Soochow University. PATU8988 cell line (5×10 6 ) were subcutaneously inoculated into the right ank of nude mice. When tumors size reached 5-10 mm in diameter, the mice were randomly divided into four different groups (n=6): Vehicle group (sesame oil), ABZ treatment group, Irradiation treatment group and ABZ and irradiation co-treatment group. As described by TC Hardin [18] , the second and fourth groups received 300 mg/kg twice daily oral gavage ABZ suspended in sesame oil, whereas the control group was treated with the vehicle control (sesame oil) for 20 days. Tumor irradiation was performed using X-RAD320iX (Precision X-Ray, North Branford, CT, USA) at a dose of 4Gy with 8.15-mA X-ray, 1.5Gy/min on days 8, 15, 22. The tumor volumes were measured every day with a vernier calipers on day 8 and were calculated using the formula: tumor volume=ab 2 /2, where "a" was the longest tumor diameter and "b" was the shortest tumor diameter measured. The PATU8988 derived tumors were harvested on the 27 th day from the mice and xed in 4% formaldehyde solution for pathological analyses or lysed in RIPA lysis buffer for western bolt analyses.
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