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Milliplex map human cardiovascular disease cvd panel 3

Manufactured by Merck Group
Sourced in United States

The Milliplex MAP Human Cardiovascular Disease (CVD) Panel 3 is a laboratory equipment product used for the multiplex detection and quantification of biomarkers related to cardiovascular disease. It is designed to measure multiple analytes simultaneously in a single sample. The core function of this product is to provide a comprehensive assessment of cardiovascular health indicators.

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2 protocols using milliplex map human cardiovascular disease cvd panel 3

1

Baseline Inflammation Biomarkers and Cancer Mortality

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In this study we examined the association between baseline circulating biomarkers for inflammation based on prior studies [26 (link)–30 (link)] and availability within the REGARDS cohort in relation to cancer mortality assessed prospectively. Biomarkers were assessed from blood samples collected during baseline physical examination, and analyzed in the REGARDS central laboratory at the University of Vermont, Burlington, VT, USA. IL-6 was measured using ultra-sensitive ELISA (Quantikine HS Human IL-6 Immunoassay, R&D Systems, Minneapolis, MN, USA); IL-8 was measured using Human Serum Adipokine Panel B LINCOplex Kit (Linco Research, Inc., St. Charles, MO, USA); and IL-10 was measured using Milliplex MAP Human Cardiovascular Disease (CVD) Panel 3 (Millipore Corporation, Billerica, MA, USA) run as a singleplex assay. A validated high-sensitivity particle-enhanced immunonephelometric assay on the BN II nephelometer (N High Sensitivity CRP, Dade Behring Inc., Deerfield, IL, USA) was used to measure CRP. The laboratory analytical intra- and inter-assay coefficient of variation (CV) for the biomarkers ranged from 1.4 to 8.1% and < 21%, respectively.
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2

Inflammatory Biomarkers Measurement Protocol

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Biomarkers of inflammation under study were interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), fibrinogen, CRP, WBC count, and cystatin C (Cys C). Blood was collected during the in-home examination after an 8- to 10-h fast; sample processing and validation of the laboratory data have been previously reported [42 (link)]. CRP and Cys C were measured by particle-enhanced immunonephelometry using the BNII nephelometer (N High Sensitivity CRP, N Latex Cystatin C; Dade Behring) [43 (link)] and WBC count was measured using an automated analyzer (Beckman Coulter, Inc., Fullerton, CA, USA) [44 (link)] at the University of Vermont Laboratory for Clinical Biochemistry Research.
IL-6, IL-8, IL-10, and fibrinogen were measured in the cohort random sample in August 2012 at the University of Vermont Laboratory for Clinical Biochemistry Research. IL-6 was measured by ultra-sensitive ELISA (Quantikine HS Human IL-6 Immunoassay; R and D Systems, Minneapolis, MN, USA), IL-8 was measured by the Human Serum Adipokine Panel B LINCOplex Kit (Linco Research, Inc.; St. Charles, MO, USA), IL-10 was measured using the Milliplex MAP Human Cardiovascular Disease (CVD) Panel 3 (Millipore Corporation; Billerica, MA, USA) run as a single-plex assay, and fibrinogen was measured using the BNII nephelometer (N Antiserum to Human Fibrinogen; Siemens Healthcare Diagnostics, Deerfield, IL, USA).
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